Utah State University, Department of Biological Engineering, 4105 Old Main Hill, Logan, UT 84322-4105, USA.
Can J Microbiol. 2014 Jul;60(7):431-5. doi: 10.1139/cjm-2013-0803. Epub 2014 May 12.
We demonstrated the production of hydrogen, ethanol, and a variety of acids by several Clostridium species using cheese whey as substrate. These species were isolated from the anaerobic sediments of a municipal wastewater stabilization pond. Eight isolates were obtained and all were classified taxonomically as Clostridium spp. based on 16S rRNA sequencing. Sludge isolates showed maximum bioproduct production yields and productivities after approximately 24 h of batch cultivation with 6% (m/v) cheese whey. Fermentation byproducts measured included hydrogen, ethanol, acetic acid, butyric acid, and lactic acid. The maximum yields of bioproducts were 0.59 mol H(2)/mol lactose, 0.071 g ethanol/g, 0.204 g acetic acid/g, 0.218 g butyric acid/g, and 0.144 g lactic acid/g. The production of these high value biofuels and biofuel intermediates from cheese whey could have significant implications for conversion of waste to high value bioproducts to enhance domestic energy economies.
我们使用奶酪乳清作为底物，展示了几种梭菌属（Clostridium）物种生产氢气、乙醇和各种酸。这些物种是从城市废水稳定塘的厌氧沉积物中分离出来的。获得了 8 个分离株，根据 16S rRNA 测序，所有分离株均被分类为梭菌属（Clostridium spp.）。在 6%（m/v）奶酪乳清的批式培养约 24 小时后，污泥分离株显示出最大的生物产物产率和生产力。测量的发酵副产物包括氢气、乙醇、乙酸、丁酸和乳酸。生物产物的最大产率为 0.59 mol H2/mol 乳糖、0.071 g 乙醇/g、0.204 g 乙酸/g、0.218 g 丁酸/g 和 0.144 g 乳酸/g。从奶酪乳清中生产这些高价值生物燃料和生物燃料中间体，对于将废物转化为高价值生物产品以增强国内能源经济具有重要意义。
