Mathieu Eva, Battiston Kyle G, McBane Joanne E, Davidson Lien, Suuronen Erik J, Santerre J Paul, Labow Rosalind S
a Division of Cardiac Surgery , University of Ottawa Heart Institute , Ottawa , Canada.
J Biomater Sci Polym Ed. 2014;25(11):1159-73. doi: 10.1080/09205063.2014.923367. Epub 2014 Jun 5.
This study investigated the interaction of human circulating angiogenic cells (CACs) with a degradable polar hydrophobic ionic polyurethane (D-PHI) which has been previously shown to exhibit anti-inflammatory character and favorable interactions with human endothelial cells (ECs). Given the implication of the CACs in microvessel development it was of intrinsic interest to expand our knowledge of D-PHI biocompatibility with this relevant primary cell involved in angiogenesis. The findings will be compared to a well-established benchmark substrate for CACs, fibronectin-coated tissue culture polystyrene (TCPS). Immunoblotting analysis showed that CACs were a heterogeneous population of cells composed mostly of monocytic cells expressing the CD14 marker. Assessment of the cytokine release profile, using ELISA, showed that D-PHI supported a higher concentration of interleukin-10 (IL-10) when compared to the concentration of tumor necrosis factor alpha, which is indicative of an anti-inflammatory phenotype, and was different from the response with TCPS. It was found that the CACs were attached to D-PHI and remained viable and functional (nitric oxide production) during the seven days of culture. However, there did not appear to be any significant proliferation on D-PHI, contrary to the CAC growth on fibronectin-coated TCPS. It was concluded that D-PHI displayed some of the qualities suitable to enable the retention of CACs onto this substrate, as well as maintaining an anti-inflammatory phenotype, characteristics which have been reported to be important for angiogenesis in vivo.
本研究调查了人循环血管生成细胞（CACs）与一种可降解的极性疏水离子聚氨酯（D-PHI）之间的相互作用，该聚氨酯先前已被证明具有抗炎特性，并与人内皮细胞（ECs）具有良好的相互作用。鉴于CACs在微血管发育中的作用，扩展我们对D-PHI与这种参与血管生成的相关原代细胞的生物相容性的认识具有内在意义。研究结果将与一种成熟的CACs基准底物——纤连蛋白包被的组织培养聚苯乙烯（TCPS）进行比较。免疫印迹分析表明，CACs是一群异质性细胞，主要由表达CD14标志物的单核细胞组成。使用酶联免疫吸附测定（ELISA）评估细胞因子释放谱，结果显示，与肿瘤坏死因子α的浓度相比，D-PHI支持更高浓度的白细胞介素-10（IL-10），这表明其具有抗炎表型，且与TCPS的反应不同。研究发现，CACs附着于D-PHI，并在培养的七天内保持存活和功能（产生一氧化氮）。然而，与在纤连蛋白包被的TCPS上生长的CACs相反，在D-PHI上似乎没有明显的增殖。研究得出结论，D-PHI表现出一些适合将CACs保留在该底物上的特性，以及维持抗炎表型，这些特性据报道对体内血管生成很重要。
