Nice E C, Simpson R J
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Victoria, Australia.
J Pharm Biomed Anal. 1989;7(9):1039-53. doi: 10.1016/0731-7085(89)80043-3.
The use of short microbore reversed phase and ion-exchange HPLC columns in the preparation of low level (submicrogram) quantities of proteins and peptides is discussed. The sequential use of columns of differing selectivity to purify complex mixtures is described. An example is given of the use of microbore columns to purify a murine myeloid leukemia inhibitory factor prior to sequence analysis.
讨论了使用短微径反相和离子交换高效液相色谱柱制备低含量（亚微克级）蛋白质和肽的方法。描述了依次使用具有不同选择性的色谱柱来纯化复杂混合物的过程。给出了一个在序列分析之前使用微径柱纯化小鼠髓样白血病抑制因子的实例。
