Food Research Laboratory, Food and Environmental Hygiene Department, 4/F Public Health Laboratory Centre, 382 Nam Cheong Street, Hong Kong SAR, China.
J Agric Food Chem. 2014 Jun 25;62(25):5805-11. doi: 10.1021/jf502326h. Epub 2014 Jun 12.
This paper reports the development of a method for the quantitative analysis of perfluorinated compounds (PFCs), including C6-C14 perfluorinated carboxylic acids (PFCAs) and C4-C12 perfluorinated sulfonates (PFSAs), in fish and fatty foods by ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) in which the UPLC was equipped a PFC Analysis Kit to eliminate background contamination. Rapid baseline separation was achieved for 17 PFCs within 12 min, and PFCs were well-resolved from potential interferences from taurodeoxycholic acid and branched isomers of PFCs. The method was validated according to Commission Regulation 2002/657/EC of the European Commission with matrices including salmon, beef, egg, and butter. Average spiked recoveries, measured at concentration levels of 0.02 (method limit of quantification (MLOQ)), 0.2, and 2 μg/kg, were in the range of 68-117% with relative standard deviations below 20%. Matrix effects were evaluated and found to be correctable by internal standardization, especially for short- and long-chained PFCs. Trueness was verified against two certified reference materials. The method has also been successfully applied to the analysis of more than 200 food samples of a risk assessment study.
本研究报告开发了一种超高效液相色谱-电喷雾串联质谱法（UPLC-ESI-MS/MS），用于定量分析鱼类和脂肪食品中的全氟化合物（PFCs），包括 C6-C14 全氟羧酸（PFCAs）和 C4-C12 全氟磺酸（PFSAs）。UPLC 配备了 PFC 分析试剂盒，以消除背景污染。17 种 PFCs 在 12 分钟内实现了快速基线分离，并且 PFCs 与牛磺脱氧胆酸和 PFCs 的支链异构体的潜在干扰物得到了很好的分离。该方法根据欧盟委员会 2002/657/EC 号委员会法规进行了验证，包括三文鱼、牛肉、鸡蛋和黄油等基质。在 0.02（方法定量下限（MLOQ））、0.2 和 2μg/kg 的浓度水平下，平均加标回收率在 68-117%范围内，相对标准偏差低于 20%。通过内标法评估了基质效应，并发现可以进行校正，特别是对于短链和长链 PFCs。通过与两种认证参考物质的比较验证了准确性。该方法还成功应用于风险评估研究中 200 多种食品样品的分析。
