Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
J Am Chem Soc. 2014 Jun 25;136(25):9225-34. doi: 10.1021/ja504213m. Epub 2014 Jun 16.
The design and construction of higher-order structure and function in proteinosome microcompartments enclosed by a cross-linked membrane of amphiphilic bovine serum albumin/poly(N-isopropylacrylamide) (BSA-NH2/PNIPAAm) nanoconjugates is described. Three structure/function relationships are investigated: (i) differential chemical cross-linking for the control of membrane disassembly and regulated release of encapsulated genetic polymers; (ii) enzyme-mediated hydrogel structuring of the internal microenvironment to increase mechanical robustness and generate a molecularly crowded reaction environment; and (iii) self-production of a membrane-enclosing outer hydrogel wall for generating protease-resistant forms of the protein-polymer protocells. Our results highlight the potential of integrating aspects of supramolecular and polymer chemistry into the design and construction of novel bioinspired microcompartments as a step toward small-scale materials systems based on synthetic cellularity.
描述了由两亲性牛血清白蛋白/聚（N-异丙基丙烯酰胺）（BSA-NH2/PNIPAAm）纳米复合物交联膜包围的蛋白体微室中高阶结构和功能的设计和构建。研究了三种结构/功能关系：（i）差异化学交联以控制膜的解体和封装遗传聚合物的调节释放；（ii）酶介导的内部微环境水凝胶结构化以增加机械强度并产生分子拥挤的反应环境；和（iii）自产生包围膜的外水凝胶壁以生成具有抗蛋白酶的蛋白-聚合物原细胞形式。我们的结果强调了将超分子和聚合物化学的各个方面整合到新型仿生微室的设计和构建中的潜力，这是朝着基于合成细胞性的小型材料系统迈出的一步。
