Di Paolo Celeste, Cabré María, Domingo José L, Gómez Mercedes
Laboratory of Toxicology and Environmental Health, School of Medicine, "Rovira i Virgili University", Sant Llorenç 21, 43201 Reus, Catalonia, Spain; Biochemical Unit, "Rovira i Virgili University", Sant Llorenç 21, 43201 Reus, Catalonia, Spain.
Biochemical Unit, "Rovira i Virgili University", Sant Llorenç 21, 43201 Reus, Catalonia, Spain.
Food Chem Toxicol. 2014 Aug;70:252-9. doi: 10.1016/j.fct.2014.05.025. Epub 2014 Jun 5.
Metals such as aluminum, iron, copper, and zinc have been implicated in the etiology of certain neurodegenerative disorders. On the other hand, it is well known that citric acid enhances Al absorption through the diet, while melatonin may bind such metals and decrease ROS production. In this study, we determined the concentrations of Al, Cu, Zn, Fe, and Mn in various tissues of Tg2576 Al-treated mice. Female mice and wild type littermates were exposed to 1mg Al/g plus 3.2% of citric acid and melatonin 10mg/kg/day for 15months. At 18months of age, metal concentrations were measured in bone, liver, kidney and spleen, as well as in three brain regions. In the citric plus Al group, Al levels were higher in hippocampus than in cortex and cerebellum, while Al concentration in bone was higher than those in kidney, liver and spleen, The current results show that exposure to Al plus citric acid did not produce relevant changes in metal levels related with genotype. Moreover, co-administration of melatonin with Al did not modify significantly metal concentrations in tissues. The present results do not support that melatonin can diminish Al or Fe concentrations in various tissues.
铝、铁、铜和锌等金属已被认为与某些神经退行性疾病的病因有关。另一方面，众所周知，柠檬酸会通过饮食增强铝的吸收，而褪黑素可能会结合此类金属并减少活性氧的产生。在本研究中，我们测定了经铝处理的Tg2576小鼠各组织中铝、铜、锌、铁和锰的浓度。雌性小鼠和野生型同窝小鼠暴露于1mg铝/克加3.2%的柠檬酸以及10mg/千克/天的褪黑素中，持续15个月。在18个月大时，测量了骨骼、肝脏、肾脏和脾脏以及三个脑区中的金属浓度。在柠檬酸加铝组中，海马体中的铝水平高于皮质和小脑，而骨骼中的铝浓度高于肾脏、肝脏和脾脏。目前的结果表明，暴露于铝加柠檬酸不会导致与基因型相关的金属水平发生相关变化。此外，褪黑素与铝共同给药并未显著改变组织中的金属浓度。目前的结果不支持褪黑素能降低各组织中铝或铁浓度的观点。
