Di Paolo Celeste, Reverte Ingrid, Colomina Maria Teresa, Domingo José L, Gómez Mercedes
Biochemistry and Biotechnology Unit, School of Medicine, IISPV, Universitat "Rovira i Virgili", Sant Llorenç 21, 43201 Reus, Catalonia, Spain; Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, Universitat "Rovira i Virgili", Sant Llorenç 21, 43201 Reus, Catalonia, Spain.
Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, Universitat "Rovira i Virgili", Sant Llorenç 21, 43201 Reus, Catalonia, Spain; Research Center for Behavior Assessment (CRAMC), Psychobiology Unit, Universitat "Rovira i Virgili", 43007 Tarragona, Catalonia, Spain.
Food Chem Toxicol. 2014 Jul;69:320-9. doi: 10.1016/j.fct.2014.04.022. Epub 2014 May 2.
Aluminum (Al) is a known neurotoxic element involved in the etiology of some serious neurodegenerative disorders such as Alzheimer disease (AD). Antioxidants like melatonin might protect neurons against the damage produced in AD. The APPSWE (Tg2576) transgenic mouse is one of the most used animal models developed to mimic AD damage. In the present study, wild type and Tg2576 mice were orally exposed during 14 months to Al, melatonin, and citric acid, as well as to all possible combinations between them. At 17 months of age, mice were evaluated for behavior using the open-field test and the Morris water maze. Transgenic animals exposed to melatonin only and to Al plus citric acid plus melatonin showed a good acquisition. No effects on acquisition in the Morris water maze were observed in wild type mice. With respect to the retention of the task, only melatonin wild type animals, and Al plus citric acid plus melatonin transgenic mice showed retention during the acquisition. Control wild type animals and Al plus citric acid plus melatonin transgenic mice showed good long term retention. Melatonin improved learning and spatial memory in Al-exposed transgenic mice.
铝（Al）是一种已知的神经毒性元素，与某些严重神经退行性疾病如阿尔茨海默病（AD）的病因有关。褪黑素等抗氧化剂可能保护神经元免受AD中产生的损伤。APPSWE（Tg2576）转基因小鼠是为模拟AD损伤而开发的最常用动物模型之一。在本研究中，野生型和Tg2576小鼠在14个月内口服暴露于铝、褪黑素、柠檬酸以及它们之间所有可能的组合。在17个月大时，使用旷场试验和莫里斯水迷宫对小鼠的行为进行评估。仅暴露于褪黑素以及暴露于铝加柠檬酸加褪黑素的转基因动物表现出良好的习得能力。在莫里斯水迷宫中，未观察到野生型小鼠的习得能力受到影响。关于任务的保持，只有褪黑素处理的野生型动物以及铝加柠檬酸加褪黑素处理的转基因小鼠在习得过程中表现出保持能力。对照野生型动物和铝加柠檬酸加褪黑素处理的转基因小鼠表现出良好的长期保持能力。褪黑素改善了铝暴露转基因小鼠的学习和空间记忆。
