人羊膜间充质基质细胞在异种淋巴细胞反应试验中对兔T细胞反应的影响。
Effects of human amniotic mesenchymal stromal cells on rabbit T-cell responses in a xenolymphocyte reaction assay.
作者信息
Xue Yifeng, Miao Zongning, Sun Hongli
机构信息
Nanjing Medical University, Affiliated Wuxi Peoples Hospital, Department of Clinical Laboratory, Wuxi, Jiangsu Province, China.
出版信息
Exp Clin Transplant. 2014 Jun;12(3):253-60.
OBJECTIVES
To evaluate the immunomodulatory properties of human amniotic mesenchymal stromal cells.
MATERIALS AND METHODS
Human amniotic mesenchymal stromal cells were isolated, characterized by flow cytometry, cultured in vitro, and evaluated in allogeneic and xenogeneic mixed lymphocyte reactions. The proliferation of T cells and the expression of interleukin 2 and interferon gamma by T cells were evaluated in the presence of human amniotic mesenchymal stromal cells.
RESULTS
Human amniotic mesenchymal stromal cells were successfully isolated from human amniotic membranes and had well-defined human mesenchymal stem cell markers (CD90, CD73, CD105, and CD166). The human amniotic mesenchymal stromal cells inhibited the proliferation of human and rabbit T cells and the secretion of interleukin-2 and interferon gamma by human T cells.
CONCLUSIONS
Human amniotic mesenchymal stromal cells may be useful for cell therapy and tissue engineering because of availability, phenotypic plasticity, and immunomodulatory properties.
目的
评估人羊膜间充质基质细胞的免疫调节特性。
材料与方法
分离人羊膜间充质基质细胞，通过流式细胞术进行表征，体外培养，并在同种异体和异种混合淋巴细胞反应中进行评估。在人羊膜间充质基质细胞存在的情况下，评估T细胞的增殖以及T细胞白细胞介素2和干扰素γ的表达。
结果
成功从人羊膜中分离出人羊膜间充质基质细胞，其具有明确的人间充质干细胞标志物（CD90、CD73、CD105和CD166）。人羊膜间充质基质细胞抑制人和兔T细胞的增殖以及人T细胞白细胞介素-2和干扰素γ的分泌。
结论
由于人羊膜间充质基质细胞易于获取、表型可塑性和免疫调节特性，其可能对细胞治疗和组织工程有用。
Zotero 插件