Poltoratsky Vladimir
Department of Biological Sciences, St. John's University, 8000 Utopia Parkway, Queens, NY, 11439, USA,
Methods Mol Biol. 2014;1172:163-71. doi: 10.1007/978-1-4939-0928-5_14.
Affinity maturation of B lymphocytes, a process that includes somatic hypermutation and class switch recombination, initiates global DNA rearrangements. The interruption of this process has an adverse effect on human health and results in immunodeficiency and autoimmune disease. Class switch recombination is a fundamental factor of the human adaptive immunity. Evaluation of the class switch recombination efficiency is an important component of laboratory diagnostic of immunotoxic components. Here, we describe a method for testing the efficiency of the class switch recombination. Cultivation of Raji Burkett's lymphoma cell line with anti-CD40 antibodies and recombinant interleukin-4 (IL-4) triggers a cascade of signal transduction network events that lead to switching the immunoglobulin isotopes from IgM to IgE. This chapter describes the methodology of class switch recombination assay for assessment of the effect of the environmental pollutants in toxicological laboratory diagnostics.
B淋巴细胞的亲和力成熟是一个包括体细胞超突变和类别转换重组的过程，它会引发全基因组DNA重排。这一过程的中断会对人类健康产生不利影响，导致免疫缺陷和自身免疫性疾病。类别转换重组是人类适应性免疫的一个基本因素。评估类别转换重组效率是免疫毒性成分实验室诊断的一个重要组成部分。在此，我们描述一种检测类别转换重组效率的方法。用抗CD40抗体和重组白细胞介素-4（IL-4）培养Raji伯基特淋巴瘤细胞系会触发一系列信号转导网络事件，从而导致免疫球蛋白同位素从IgM转换为IgE。本章描述了用于评估环境污染物在毒理学实验室诊断中作用的类别转换重组测定方法。
