Rollins Janet, Miskolci Veronika
Division of Natural Sciences, College of Mount Saint Vincent, 6301 Riverdale Avenue, Riverdale, NY, 10471, USA,
Methods Mol Biol. 2014;1172:263-70. doi: 10.1007/978-1-4939-0928-5_24.
Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1-2 days.
免疫荧光是观察细胞内蛋白质表达、定位和共定位所需的一项重要技术。在此，我们描述了人中性粒细胞（多形核白细胞；PMN）中肿瘤坏死因子-α（TNF）的免疫荧光及后续共聚焦显微镜技术。该定性技术可用于观察静息白细胞与活化白细胞的表达模式变化。还可观察其与其他细胞因子、蛋白质或细胞器的共定位情况。这项免疫荧光技术可在1至2天内完成。
