College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China.
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China.
Plant Sci. 2014 Jul;224:120-35. doi: 10.1016/j.plantsci.2014.04.012. Epub 2014 Apr 26.
Maturation of fruits involves a series of physiological, biochemical, and organoleptic changes that eventually make fleshy fruits attractive, palatable, and nutritional. In order to understand the mature mechanism of the early-maturing bud sport of 'Zaosu' pear, we analyzed the differences of proteome expression between the both pears in different mature stages by the methods of a combination of two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Seventy-five differential expressed protein spots (p<0.05) were obtained between 'Zaosu' pear and its early-maturing bud sport, but only sixty-eight were demonstratively identified in the database of NCBI and uniprot. The majority of proteins were linked to metabolism, energy, stress response/defense and cell structure. Additionally, our data confirmed an increase of proteins related to cell-wall modification, oxidative stress and pentose phosphate metabolism and a decrease of proteins related to photosynthesis and glycolysis during the development process of both pears, but all these proteins increased or decreased faster in the early-maturing bud sport. This comparative analysis between both pears showed that these proteins were closely associated with maturation and could provide more detailed characteristics of the maturation process of both pears.
果实的成熟过程涉及一系列生理、生化和感官变化，最终使肉质果实具有吸引力、可口和营养。为了了解“早酥”梨早熟芽变的成熟机制，我们采用二维电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析相结合的方法，分析了不同成熟阶段两品种梨之间蛋白质组表达的差异。在“早酥”梨与其早熟芽变之间获得了 75 个差异表达的蛋白质斑点（p<0.05），但只有 68 个在 NCBI 和 uniprot 数据库中得到了明确鉴定。大多数蛋白质与代谢、能量、应激反应/防御和细胞结构有关。此外，我们的数据还证实，在两品种梨的发育过程中，与细胞壁修饰、氧化应激和戊糖磷酸代谢相关的蛋白质增加，与光合作用和糖酵解相关的蛋白质减少，但所有这些蛋白质在早熟芽变中增加或减少得更快。两品种梨之间的比较分析表明，这些蛋白质与成熟密切相关，可以为两品种梨的成熟过程提供更详细的特征。
