Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas, Campus Rabanales, Edif. Severo Ochoa, 1ª Planta, Universidad de Córdoba, 14071 Córdoba, Spain.
Departamento de Botánica, Ecología y Fisiología Vegetal, Grupo de Fisiología Molecular y Biotecnología de Plantas, Campus Rabanales, Edif. Severo Ochoa, 1ª Planta, Universidad de Córdoba, 14071 Córdoba, Spain.
Plant Sci. 2014 Jul;224:137-43. doi: 10.1016/j.plantsci.2014.04.017. Epub 2014 May 4.
Plant nucleases are involved in nucleic acid degradation associated to programmed cell death processes as well as in DNA restriction, repair and recombination processes. However, the knowledge about the function of plant nucleases is limited. A major nuclease activity was detected by in-gel assay with whole embryonic axes of common bean by using ssDNA or RNA as substrate, whereas this activity was minimal in cotyledons. The enzyme has been purified to electrophoretic homogeneity from embryonic axes. The main biochemical properties of the purified enzyme indicate that it belongs to the S1/P1 family of nucleases. This was corroborated when this protein, after SDS-electrophoresis, was excised from the gel and further analysis by MALDI TOF/TOF allowed identification of the gene (PVN1) that codes this protein. The gene that codes the purified protein was identified. The expression of PVN1 gene was induced at the specific moment of radicle protrusion. The inclusion of inorganic phosphate to the imbibition media reduced the level of expression of this gene and the nuclease activity suggesting a relationship with the phosphorous status in French bean seedlings.
植物核酸酶参与与程序性细胞死亡过程相关的核酸降解以及 DNA 限制、修复和重组过程。然而，人们对植物核酸酶的功能知之甚少。通过使用 ssDNA 或 RNA 作为底物的凝胶内测定，在普通菜豆的整体胚胎轴中检测到主要的核酸酶活性，而在子叶中这种活性最小。该酶已从胚胎轴中电泳纯化为均一。纯化酶的主要生化特性表明它属于 S1/P1 家族的核酸酶。当该蛋白在 SDS-电泳后从凝胶中切出并用 MALDI TOF/TOF 进一步分析时，证实了这一点，从而鉴定出编码该蛋白的基因 (PVN1)。鉴定出了编码纯化蛋白的基因。PVN1 基因的表达在胚根突出的特定时刻被诱导。向吸胀培养基中添加无机磷酸盐会降低该基因的表达水平和核酸酶活性，表明其与法国菜豆幼苗的磷状态有关。
