Departamento de Odontopediatria e Ortodontia, Faculdade de Odontologia, UFRJ, Rio de Janeiro, Brazil.
Lett Appl Microbiol. 2014 Sep;59(3):342-7. doi: 10.1111/lam.12281. Epub 2014 Jun 7.
This study determined the changes of calcium concentration in a medium containing teeth/biofilm exposed to Coffea canephora extract (CCE). Enamel fragments were randomly fixed into two 24-well polystyrene plates containing BHI. Pooled human saliva was added to form biofilm on fragments. Specimens were divided into treatment groups (G, n = 8 per group) and treated with 50 μl daily for 1 min per week, as follows: G1, 20% CCE; G2, Milli-Q water (negative control); G3, antibiotic (positive control). Six fragments represented the blank control (G4). The calcium content was observed at baseline, 4 and 7 days of treatment by atomic-absorption spectrophotometry. Cross-sectional hardness of enamel was a demineralization indicator. Calcium increased in the medium after 4 and 7 days of treatment in G1 (3·80 ± 1·3 mg l(-1) and 4·93 ± 2·1 mg l(-1) , respectively) and G3 (4th day = 5·7 ± 1·8 mg l(-1) ; 7th day = 6·7 ± 3·5 mg l(-1) ) (P > 0·05). Calcium from G2 decreased after 7 days, which was different from G3 (P < 0·05). The lower calcium content, at the end of the experiment, was represented by G4, 2·16 ± 0·2 mg l(-1) . The increase in calcium after treatment with CCE is probably due to its antibacterial effect, which caused the bacterial lysis and consequent release of calcium in the medium.
This study revealed an inhibitory action of Coffea canephora against dental biofilm. This coffee species caused bacterial lysis and consequent release of calcium into the medium. Furthermore, the advantage of coffee as an antibacterial beverage is that it is consumed in a concentrated form (6-10%) as opposed to various medicinal infusions that have shown such effect in vitro and are usually consumed at 1-2%. Therefore, a light roasted C. canephora aqueous extract can be considered as a potential anticariogenic substance.
本研究旨在确定含有牙齿/生物膜的培养基中钙浓度的变化，这些牙齿/生物膜暴露于咖啡（Coffea canephora）提取物（CCE）下。方法：随机将牙釉质碎片固定在含有 BHI 的两个 24 孔聚苯乙烯板中。将混合唾液添加到碎片上以形成生物膜。将标本分为治疗组（G，每组 8 个标本），并每周每天处理 1 分钟，用 50 μl 处理，如下所示：G1，20%CCE；G2，Milli-Q 水（阴性对照）；G3，抗生素（阳性对照）。六个碎片代表空白对照（G4）。通过原子吸收分光光度法在基线、治疗后 4 天和 7 天观察钙含量。牙釉质的横截面硬度是脱矿指示。结果：在 G1（分别为 3·80±1·3 mg l（-1）和 4·93±2·1 mg l（-1））和 G3（第 4 天=5·7±1·8 mg l（-1）；第 7 天=6·7±3·5 mg l（-1））中，处理后培养基中的钙增加（P>0·05）。G2 的钙含量在第 7 天减少，与 G3 不同（P<0·05）。实验结束时，G4 的钙含量较低，为 2·16±0·2 mg l（-1）。CCE 处理后钙含量增加可能是由于其抗菌作用，导致细菌裂解，继而将钙释放到培养基中。结论：本研究揭示了咖啡（Coffea canephora）对牙生物膜的抑制作用。这种咖啡种类导致细菌裂解，继而将钙释放到培养基中。此外，咖啡作为一种抗菌饮料的优势在于，它以浓缩形式（6-10%）消费，而不是各种已证明具有体外这种作用且通常以 1-2%消费的药用汤剂。因此，轻度烘焙的 C. canephora 水提取物可以被认为是一种有潜力的抗龋物质。
