Bernstein Joseph A, Frank Linda A, Kania Stephen A
Long Green Animal Dermatology Center, 13515 Long Green Pike, Baldwin, MD, 21013, USA.
Vet Dermatol. 2014 Oct;25(5):487-e80. doi: 10.1111/vde.12141. Epub 2014 Jun 9.
Molecular characterization of Demodex mites is being used to identify mite species in dogs. This technique is now being applied to cat Demodex species, allowing for better characterization of the mites.
HYPOTHESIS/OBJECTIVES: Molecular diagnostics will clarify the existence of diverse Demodex mites identified morphologically.
A cat with generalized demodicosis secondary to chronic steroid treatment for erythroid dysplasia.
Skin scrapings demonstrated large numbers of follicular mites consistent with Demodex cati as well as a morphologically different Demodex mite with a blunted abdomen. The 16S rRNA DNA was amplified by PCR, sequenced and compared with available Demodex sequences, including Demodex cati, Demodex gatoi and an unnamed Demodex sp.
A single PCR product was obtained, the DNA sequence of which was an exact match with D. cati.
The shorter unnamed mite was not a different species in this case, but a different morphological form of D. cati. This report demonstrates the utility of molecular diagnostics to clarify the identity of mites that differ morphologically.
蠕形螨的分子特征分析正被用于鉴定犬类中的螨种。这项技术目前正应用于猫蠕形螨种，以便更好地对螨进行特征描述。
假设/目的：分子诊断将阐明形态学上鉴定出的多种蠕形螨的存在情况。
一只因红细胞发育异常接受慢性类固醇治疗继发全身性蠕形螨病的猫。
皮肤刮片显示大量与猫蠕形螨一致的毛囊螨，以及一种腹部钝圆、形态不同的蠕形螨。通过聚合酶链反应（PCR）扩增16S rRNA DNA，进行测序，并与现有的蠕形螨序列进行比较，包括猫蠕形螨、猫背肛蠕形螨和一种未命名的蠕形螨种。
获得了单一的PCR产物，其DNA序列与猫蠕形螨完全匹配。
在这种情况下，较短的未命名螨不是一个不同的物种，而是猫蠕形螨的一种不同形态。本报告证明了分子诊断在阐明形态不同的螨的身份方面的实用性。
