含多个十二烷基磷酸三酯键的两亲性DNA与脂质双分子层膜结合特性的调控

Modulation of binding properties of amphiphilic DNA containing multiple dodecyl phosphotriester linkages to lipid bilayer membrane.

作者信息

Makishi Shingo, Shibata Tomonori, Okazaki Masatsugu, Dohno Chikara, Nakatani Kazuhiko

机构信息

Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research (ISIR), Osaka University, Ibaraki 567-0047, Japan.

Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research (ISIR), Osaka University, Ibaraki 567-0047, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.

出版信息

Bioorg Med Chem Lett. 2014 Aug 1;24(15):3578-81. doi: 10.1016/j.bmcl.2014.05.042. Epub 2014 May 23.

DOI:10.1016/j.bmcl.2014.05.042

PMID:24909080

Abstract

DNA is a promising functional molecule to modify and design lipid membrane functions. In order to use DNA in a hydrophilic-hydrophobic interface including lipid membrane, we have developed an amphiphilic DNA having dodecyl phosphotriester linkages (dod-DNA). Herein, we report the binding of a series of amphiphilic dod-DNAs to the lipid bilayer membrane. Surface plasmon resonance (SPR) assay and fluorescent microscopy showed that dod-DNA having three dodecyl groups at each end strongly bound to lipid membrane due to the slow dissociation rate and the dod-DNA can be used as a linear template for molecular arrangement on the membrane surface.

摘要

DNA是一种用于修饰和设计脂质膜功能的很有前景的功能分子。为了在包括脂质膜的亲水-疏水界面中使用DNA，我们开发了一种具有十二烷基磷酸三酯键的两亲性DNA（dod-DNA）。在此，我们报道了一系列两亲性dod-DNA与脂质双层膜的结合。表面等离子体共振（SPR）分析和荧光显微镜显示，两端各有三个十二烷基的dod-DNA由于解离速率慢而与脂质膜紧密结合，并且dod-DNA可以用作膜表面分子排列的线性模板。