Lehrstuhl für Systembiophysik, Physik-Department - E14 und ZNN-WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching (Germany) http://www.e14.ph.tum.de.
Angew Chem Int Ed Engl. 2014 Apr 14;53(16):4236-9. doi: 10.1002/anie.201310259. Epub 2014 Mar 11.
Amphiphilic compounds have a strong tendency to form aggregates in aqueous solutions. It is shown that such aggregation can be utilized to fold cholesterol-modified, single-layered DNA origami structures into sandwich-like bilayer structures, which hide the cholesterol modifications in their interior. The DNA bilayer structures unfold after addition of the surfactant Tween 80, and also in the presence of lipid bilayer membranes, with opening kinetics well described by stretched exponentials. It is also demonstrated that by combination with an appropriate lock and key mechanism, hydrophobic actuation of DNA sandwiches can be made conditional on the presence of an additional molecular input such as a specific DNA sequence.
两亲化合物在水溶液中具有强烈的形成聚集物的趋势。研究表明，这种聚集可以用于将胆固醇修饰的单层 DNA 折纸结构折叠成夹心双层结构，从而将胆固醇修饰隐藏在其内部。在添加表面活性剂 Tween 80 后，DNA 双层结构会展开，并且在存在脂质双层膜的情况下，其打开动力学可以很好地用拉伸指数函数来描述。还证明了，通过与适当的锁和钥匙机制相结合，疏水驱动的 DNA 三明治的激活可以取决于附加的分子输入，例如特定的 DNA 序列。
