Lai Wenying, Wu Weifeng, Pan Xiaofen, Kong Qing
Department of Cardiology, Guangxi Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jun;30(6):601-3, 607.
To explore the effect of interleukin-17A (IL-17A) on the serum level of antiheart autoantibodies in mice with viral myocarditis.
Male wild-type (WT) and IL-17A-deficient (IL-17A(-/-)) BALB/c mice were intraperitoneally injected with Coxsackie virus B3 (CVB3) for establishing VMC models (VMC-WT group and VMC-IL-17A(-/-) group). Meanwhile, a control group (WT group) of WT mice were established by i.p. administration of phosphate buffered saline (PBS). Paraffin sections of cardiac tissues were made 14 days after CVB3 injection. Myocardial histopathologic changes were evaluated by HE staining. The levels of anti-adenine nucleotide translocator (ANT) autoantibody, anti-β-myosin heavy chain (β-MHC) autoantibody and anti-cardiac L-type calcium channel (CACH2) autoantibody in sera were measured by ELISA.
Compared with WT group, the levels of anti-ANT-autoantibody and anti-β-MHC-autoantibody significantly increased in VMC-WT group (P<0.01, P<0.05), while the concentration of anti-CACH2-autoantibody showed no significant difference between WT and VMC-WT groups (P>0.05). Compared with VMC-WT group, the level of anti-ANT-autoantibody was reduced in VMC-IL-17A(-/-) group (P<0.05), while the levels of anti-β-MHC-autoantibody and anti-CACH2-autoantibody showed no significant difference between them (P>0.05).
IL-17A contributed to the secretion of anti-ANT-autoantibody of VMC mice, but had no effect on the secretion of anti-β-MHC-autoantibody and anti-CACH2-autoantibody in VMC mice.
探讨白细胞介素-17A（IL-17A）对病毒性心肌炎小鼠血清抗心脏自身抗体水平的影响。
雄性野生型（WT）和IL-17A基因敲除（IL-17A(-/-)）BALB/c小鼠腹腔注射柯萨奇病毒B3（CVB3）建立病毒性心肌炎模型（VMC-WT组和VMC-IL-17A(-/-)组）。同时，野生型小鼠对照组（WT组）腹腔注射磷酸盐缓冲液（PBS）。CVB3注射14天后制作心脏组织石蜡切片。通过HE染色评估心肌组织病理学变化。采用酶联免疫吸附测定法检测血清中抗腺嘌呤核苷酸转位酶（ANT）自身抗体、抗β-肌球蛋白重链（β-MHC）自身抗体和抗心脏L型钙通道（CACH2）自身抗体水平。
与WT组相比，VMC-WT组抗ANT自身抗体和抗β-MHC自身抗体水平显著升高（P<0.01，P<0.05），而WT组和VMC-WT组抗CACH2自身抗体浓度无显著差异（P>0.05）。与VMC-WT组相比，VMC-IL-17A(-/-)组抗ANT自身抗体水平降低（P<0.05），而抗β-MHC自身抗体和抗CACH2自身抗体水平无显著差异（P>0.05）。
IL-17A促进病毒性心肌炎小鼠抗ANT自身抗体的分泌，但对病毒性心肌炎小鼠抗β-MHC自身抗体和抗CACH2自身抗体的分泌无影响。
