基于扩增阻滞突变系统的ABO血型基因分型
[ABO blood group genotyping based on amplification refractory mutation system].
作者信息
Zhang Chao, Zhu Juanli, Yang Jiangcun, Ma Ting, Hui Wenli, Cui Yali
机构信息
College of Life Sciences, Northwest University, Xi'an 710069, China.
National Engineering Research Center for Miniaturized Detection Systems, Xi'an 710069, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jun;30(6):643-6.
OBJECTIVE
To develop a rapid, cost-effective and reliable method for ABO genotyping.
METHODS
Six pairs of primers were designed for the amplification refractory mutation system (ARMS). Thirty peripheral blood DNA samples, derived from Chinese Han unrelated individuals, were genotyped by ARMS. The results were compared with the corresponding data obtained from serological methods and DNA sequencing.
RESULTS
The ABO genotyping results using ARMS were perfectly in line with the serological methods and DNA sequencing.
CONCLUSION
The method developed and optimized in this study is able to accurately and rapidly detect 28 kinds of ABO genotypes, indicative of a new approach applicable to clinical practice.
目的
开发一种快速、经济高效且可靠的ABO基因分型方法。
方法
设计了6对引物用于扩增阻滞突变系统（ARMS）。采用ARMS对30份来自中国汉族无关个体的外周血DNA样本进行基因分型。将结果与血清学方法和DNA测序获得的相应数据进行比较。
结果
使用ARMS进行ABO基因分型的结果与血清学方法和DNA测序完全一致。
结论
本研究开发并优化的方法能够准确、快速地检测28种ABO基因型，表明这是一种适用于临床实践的新方法。
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