人头皮毛囊球部三磷酸腺苷（ATP）含量的评估

Evaluation of ATP content in hair bulbs in human scalp.

作者信息

Mikhal'chik E V, Suprun M V, Fedorkova M V, Ibragimova G A, Dmitrieva E I, Lipatova V A, Kutsev S I

机构信息

Laboratory of Biophysical Methods of Diagnosis, Research Institute of Physico-Chemical Medicine, Federal Medical and Biological Agency of the Russian Federation, Moscow, Russia,

出版信息

Bull Exp Biol Med. 2014 May;157(1):112-5. doi: 10.1007/s10517-014-2504-2. Epub 2014 Jun 10.

DOI:10.1007/s10517-014-2504-2

PMID:24909726

Abstract

The content of ATP in scalp hair bulbs in humans was measured in the hair roots from 15 healthy volunteers. Light and electron microscopy confirmed the presence of outer and an inner root sheaths in the root of pulled out anagen hair. Incubation of samples in buffer solution led to extraction of ATP, which was measured by the chemiluminescent method. Mechanic disintegration of hair bulbs and their freezing-defrosting did not increase ATP output. The results of microscopy indicated that ATP extraction procedure was associated with separation of the outer radical sheath from the inner one without impairing the structure of the inner sheath. The mean content of ATP was 12 ± 2 pmol per bulb. The use of pulled out hair bulbs for ATP measurements simplified the procedure as involved no surgical removal of follicles.

摘要

对15名健康志愿者发根的头皮毛囊球中的三磷酸腺苷（ATP）含量进行了测定。光学显微镜和电子显微镜证实，拔出的生长期毛发根部存在外根鞘和内根鞘。将样本在缓冲溶液中孵育可提取ATP，通过化学发光法进行测定。毛囊球的机械分解及其冻融处理并未增加ATP的产量。显微镜检查结果表明，ATP提取过程与外根鞘与内根鞘的分离有关，而不会损害内根鞘的结构。每个毛囊球中ATP的平均含量为12±2皮摩尔。使用拔出的毛囊球进行ATP测量简化了操作程序，因为无需手术切除毛囊。