Federal Research Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Moscow Practical Center of Dermatovenerology and Cosmetology, Moscow Healthcare Department, Moscow, Russia.
Bull Exp Biol Med. 2021 Jan;170(3):299-302. doi: 10.1007/s10517-021-05055-0. Epub 2021 Jan 16.
The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length <1 mm and ATP content surpassed the normal in follicles >2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.
从健康志愿者（正常值）和雄激素性脱发患者的拔取的毛囊提取物中测量了 ATP、IL-6 和 IL-10 的浓度，然后为每个毛囊计算了 ATP、IL-6 和 IL-10 的含量。除了 IL-6 之外，这些值与毛囊长度成正比。提取的 ATP 浓度与乳酸脱氢酶活性相关，表明细胞损伤。在雄激素性脱发患者中，IL-10 含量在长度 <1 毫米的毛囊中超过正常值，而 ATP 含量在长度 >2 毫米的毛囊中超过正常值。通过 ELISA 测量的 IL-6 和 IL-10 含量与通过 RT-PCR 测定的 mRNA 表达结果相当，这证明了基因表达的中等水平。ATP 和 IL-10 的含量（但不是 IL-6）取决于拔取的毛囊的长度和影响头发生长的致病因素。
