Rejholcová M, Wilhelm J
Acta Univ Carol Med (Praha). 1989;35(1-2):43-61.
The present work was aimed at investigation of lipid peroxidation during fasting and its effects upon metabolism of adipose tissue, especially lipolytic activity. An increase in the lipid peroxidation was demonstrated in an animal model (male rats) through accumulation of lipofuscin-like pigments (LFP), the end product of lipid peroxidation. The increased LFP levels correlated with a decrease in the activity of hormone-sensitive lipase. LFP content increased in the plasma, liver and adipose tissue. The time-course of the changes depended on the initial body mass of the animals. Correlations were found between lipolytic activity and LFP content in adipose tissue and in plasma. In the course of the repeated cycles of fasting--feeding, the changes of LFP and lipolysis were antiparallel to each other. During four cycles, there was a net increase in LFP and a net decrease in lipolysis. The lipolytic activity depended on many factors, among which we identified the initial body mass of animals, their age, and the way of feeding. The results obtained in this animal model enable application of the methods used in the investigation of fasting in obese patients.
本研究旨在探讨禁食期间的脂质过氧化及其对脂肪组织代谢的影响，尤其是脂解活性。通过脂质过氧化终产物脂褐素样色素（LFP）的积累，在动物模型（雄性大鼠）中证实了脂质过氧化增加。LFP水平升高与激素敏感性脂肪酶活性降低相关。血浆、肝脏和脂肪组织中的LFP含量增加。变化的时间进程取决于动物的初始体重。在脂肪组织和血浆中发现脂解活性与LFP含量之间存在相关性。在禁食-进食的重复周期过程中，LFP和脂解的变化彼此呈反平行。在四个周期中，LFP净增加，脂解净减少。脂解活性取决于许多因素，其中我们确定了动物的初始体重、年龄和喂养方式。在该动物模型中获得的结果使得能够应用于肥胖患者禁食研究中所使用的方法。
