Masood Nusrat, Luqman Suaib
Molecular Bioprospection Department, Biotechnology Division, CSIR-Central Institute of Medicinal & Aromatic Plants, Lucknow-226015, India.
Comb Chem High Throughput Screen. 2014;17(8):718-22. doi: 10.2174/1386207317666140605110401.
A 2, 2`azino-bis-(3-ethyl-benzothiazoline-6-sulphonate) radical cation-based method was optimized in 96 well plates to evaluate the comparative scavenging potential of phenolic and non-phenolic group of molecules with respect to control. The interactions of these molecules with ABTS radical cation were quantified on the basis of their relative influence on the bleaching of a bluish-green color complex in a structure- and dose-dependent manner. Experimentally, the developed assay provided evidence that phenolic molecules are more reactive with ABTS radical than non-phenolic compounds because of their resonance and hyper-conjugation effects.
在96孔板中优化了基于2,2'-偶氮双-(3-乙基苯并噻唑啉-6-磺酸盐)自由基阳离子的方法，以评估酚类和非酚类分子相对于对照的比较清除潜力。这些分子与ABTS自由基阳离子的相互作用根据它们对蓝绿色络合物漂白的相对影响以结构和剂量依赖的方式进行定量。实验表明，由于酚类分子的共振和超共轭效应，它们与ABTS自由基的反应性比非酚类化合物更高。
