Bhattacharya Abhishek, Pletschke Brett I
Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown 6140, South Africa.
Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown 6140, South Africa.
Enzyme Microb Technol. 2014 Jul-Aug;61-62:17-27. doi: 10.1016/j.enzmictec.2014.04.009. Epub 2014 Apr 28.
The enzymatic conversion of lignocellulosic biomass into biofuels has been identified as an excellent strategy to generate clean energy. However, the current process is cost-intensive as an effective immobilization approach to reuse the enzyme(s) has been a major challenge. The present study introduces the concept and application of novel magnetic cross-linked enzyme aggregates (mag-CLEAs). Both mag-CLEAs and calcium-mag-CLEAs (Ca-mag-CLEAs) exhibited a 1.35 fold higher xylanase activity compared to the free enzyme and retained more than 80.0% and 90.0% activity, respectively, after 136h of incubation at 50°C, compared to 50% activity retained by CLEAs. A 7.4 and 9.0 fold higher sugar release from lime-pretreated and NH4OH pre-treated sugar bagasse, respectively, was achieved with Ca-mag-CLEAs compared to the free enzymes. The present study promotes the successful application of mag-CLEAs and Ca-mag-CLEAs as carrier free immobilized enzymes for the effective hydrolysis of lignocellulolytic biomass and associated biofuel feedstocks.
将木质纤维素生物质酶转化为生物燃料已被视为一种生产清洁能源的卓越策略。然而，当前工艺成本高昂，因为找到一种有效的固定化方法以重复利用酶一直是一项重大挑战。本研究介绍了新型磁性交联酶聚集体（mag-CLEAs）的概念及应用。与游离酶相比，mag-CLEAs和钙-磁性交联酶聚集体（Ca-mag-CLEAs）的木聚糖酶活性均高出1.35倍，并且在50°C孵育136小时后，分别保留了超过80.0%和90.0%的活性，而交联酶聚集体（CLEAs）仅保留了50%的活性。与游离酶相比，Ca-mag-CLEAs分别使经石灰预处理和NH4OH预处理的甘蔗渣的糖释放量提高了7.4倍和9.0倍。本研究推动了mag-CLEAs和Ca-mag-CLEAs作为无载体固定化酶在木质纤维素生物质及相关生物燃料原料有效水解中的成功应用。
