Int J Phytoremediation. 2014;16(2):123-37. doi: 10.1080/15226514.2012.759532.
Effect of nitric oxide (NO) in mitigating stress induced by arsenic (As) was assessed in Pistia stratiotes, with NO supplied as sodium nitroprusside (SNP). Plants were exposed to four treatments: control, SNP (0.1 mg L(-1)), As (1.5 mg L(-1)), As + SNP (1.5 and 0.1 mg L(-1)), for seven days (analyses of growth, absorption of As and mineral nutrients) and for 24 h (analyses of concentration of reactive oxygen intermediates (ROIs), antioxidant capacity and photosynthesis). P. stratiotes accumulated high concentrations of As and this accumulation wasn't affected by the addition of SNP, but the tolerance index of the plant to As increased. SNP attenuated effects of As on the absorption of mineral nutrients (Ca, Fe, Mn, and Mg), but not for phosphorus, and maintained concentrations of ROIs to normal levels, probably due to the increase in antioxidant capacity. The As damaged the photosynthesis by the decrease in pigment contents and by disturbance the photochemical (loss of PSII efficiency and increases in non-photochemical quenching coefficient) and biochemical (reductions in carbon assimilation, increase in the C(i)/C(a) and phi(PSII)/phi(CO2) ratios) steps. The addition of SNP restored these parameters to normal levels. Thus, NO was able to increasing the resistance of P. stratiotes to As.
评估了一氧化氮（NO）通过提供硝普酸钠（SNP）来减轻砷（As）引起的胁迫对浮萍的影响。将植物暴露于四种处理：对照、SNP（0.1 mg L（-1））、As（1.5 mg L（-1））、As + SNP（1.5 和 0.1 mg L（-1）），进行七天（生长、As 和矿物质养分吸收分析）和 24 小时（活性氧中间体（ROIs）浓度、抗氧化能力和光合作用分析）。浮萍积累了高浓度的 As，并且 SNP 的添加并没有影响 As 的积累，但植物对 As 的耐受指数增加。SNP 减轻了 As 对矿物质养分（Ca、Fe、Mn 和 Mg）吸收的影响，但对磷没有影响，并且保持 ROIs 的浓度正常，可能是由于抗氧化能力的增加。As 通过降低色素含量和干扰光化学（PSII 效率降低和非光化学猝灭系数增加）和生化（碳同化减少、C（i）/C（a）和 phi（PSII）/phi（CO2）比值增加）步骤破坏光合作用。添加 SNP 将这些参数恢复到正常水平。因此，NO 能够提高浮萍对 As 的抗性。
