Antu Kalathookunnel Antony, Riya Mariam Philip, Mishra Arvind, Sharma Sharad, Srivastava Arvind K, Raghu Kozhiparambil Gopalan
Agroprocessing and Natural Products Division, Council of Scientific and Industrial Research - National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram 695019, Kerala, India.
Division of Biochemistry, CSIR - Central Drug Research Institute (CSIR-CDRI), Lucknow 226001, Uttar Pradesh, India.
Exp Toxicol Pathol. 2014 Sep;66(7):281-91. doi: 10.1016/j.etp.2014.05.004. Epub 2014 Jun 6.
The beneficial effects of hydroethanol extract of Symplocos cochinchinensis (SCE) has been explored against hyperglycemia associated secondary complications in streptozotocin induced diabetic rat model. The experimental groups consist of normal control (NC), diabetic control (DC), DC + metformin 100 mg kg(-1) bwd, DC + SCE 250 and DC + SCE 500. SCEs and metformin were administered daily for 21 days and sacrificed on day 22. Oral glucose tolerance test, plasma insulin, % HbA1c, urea, creatinine, aspartate aminotransferase, alanine aminotransferase, albumin, total protein etc. were analysed. Aldose reductase (AR) activity in the eye lens was also checked. On day 21, DC rats showed significantly abnormal glucose response, HOMA-IR, % HbA1c, decreased activity of antioxidant enzymes and GSH, elevated AR activity, hepatic and renal oxidative stress markers like malondialdehyde, protein carbonyls compared to NC. DC rats also exhibited increased level of plasma urea and creatinine. Treatment with SCE protected from the deleterious alterations of biochemical parameters in a dose dependent manner including histopathological alterations in pancreas. SCE 500 exhibited 46.28% of glucose lowering effect and decreased HOMA-IR (2.47), % HbA1c (6.61), lens AR activity (15.99%), and hepatic, renal oxidative stress and function markers compared to DC group. Considerable amount of liver and muscle glycogen was replenished by SCE treatment in diabetic animals. Although metformin showed better effect, the activity of SCE was very much comparable with this drug.
已在链脲佐菌素诱导的糖尿病大鼠模型中探究了山矾叶乙醇提取物（SCE）对高血糖相关继发并发症的有益作用。实验组包括正常对照组（NC）、糖尿病对照组（DC）、DC + 二甲双胍100 mg kg⁻¹体重、DC + SCE 250和DC + SCE 500。每天给予SCE和二甲双胍，持续21天，并在第22天处死。分析了口服葡萄糖耐量试验、血浆胰岛素、糖化血红蛋白百分比、尿素、肌酐、天冬氨酸转氨酶、丙氨酸转氨酶、白蛋白、总蛋白等。还检测了晶状体中的醛糖还原酶（AR）活性。在第21天，与NC组相比，DC大鼠表现出明显异常的葡萄糖反应、稳态模型评估胰岛素抵抗（HOMA-IR）、糖化血红蛋白百分比、抗氧化酶和谷胱甘肽活性降低、AR活性升高、肝和肾氧化应激标志物如丙二醛、蛋白质羰基含量增加。DC大鼠还表现出血浆尿素和肌酐水平升高。SCE治疗以剂量依赖方式保护生化参数免受有害改变，包括胰腺的组织病理学改变。与DC组相比，SCE 500表现出46.28%的降糖作用，并降低了HOMA-IR（2.47）、糖化血红蛋白百分比（6.61）、晶状体AR活性（15.99%）以及肝、肾氧化应激和功能标志物。SCE治疗使糖尿病动物的肝脏和肌肉糖原得到大量补充。尽管二甲双胍显示出更好的效果，但SCE的活性与该药物非常相当。
