Low Van Lun, Tan Tiong Kai, Lim Phaik Eem, Domingues Luísa Nogueira, Tay Sun Tee, Lim Yvonne Ai Lian, Goh Thary Gazi, Panchadcharam Chandrawathani, Bathmanaban Premaalatha, Sofian-Azirun Mohd
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Vet Parasitol. 2014 Aug 29;204(3-4):439-42. doi: 10.1016/j.vetpar.2014.05.036. Epub 2014 May 29.
A multilocus sequence analysis using mitochondria-encoded cytochrome c oxidase subunit I (COI), cytochrome B (CytB), NADH dehydrogenase subunit 5 (ND5); nuclear encoded 18S ribosomal RNA (18S) and 28S ribosomal RNA (28S) genes was performed to determine the levels of genetic variation between the closely related species Haematobia irritans Linnaeus and Haematobia exigua de Meijere. Among these five genes, ND5 and CytB genes were found to be more variable and informative in resolving the interspecific relationships of both species. In contrast, the COI gene was more valuable in inferring the intraspecific relationships. The ribosomal 18S and 28S sequences of H. irritans and H. exigua were highly conserved with limited intra- and inter-specific variation. Molecular evidence presented in this study demonstrated that both flies are genetically distinct and could be differentiated based on sequence analysis of mitochondrial genes.
进行了一项多位点序列分析，使用线粒体编码的细胞色素c氧化酶亚基I（COI）、细胞色素B（CytB）、NADH脱氢酶亚基5（ND5）；核编码的18S核糖体RNA（18S）和28S核糖体RNA（28S）基因，以确定近缘物种嗜人血蝇（Haematobia irritans Linnaeus）和微小血蝇（Haematobia exigua de Meijere）之间的遗传变异水平。在这五个基因中，发现ND5和CytB基因在解析两个物种的种间关系方面更具变异性和信息性。相比之下，COI基因在推断种内关系方面更有价值。嗜人血蝇和微小血蝇的核糖体18S和28S序列高度保守，种内和种间变异有限。本研究提供的分子证据表明，这两种苍蝇在基因上是不同的，可以根据线粒体基因的序列分析进行区分。
