朝藿定与灯盏花素合用可改善自发性高血压大鼠的勃起功能。

Icariin combined with breviscapine improves the erectile function of spontaneously hypertensive rats.

机构信息

Department of Cardiovascular Diseases, Affiliated Hospital, Luzhou Medical College, Luzhou, China.

出版信息

J Sex Med. 2014 Sep;11(9):2143-52. doi: 10.1111/jsm.12614. Epub 2014 Jun 10.

DOI:10.1111/jsm.12614

PMID:24912989

Abstract

INTRODUCTION

The impaired erectile response in spontaneously hypertensive rats (SHR) is caused by increased signaling of RhoA/Rho-kinase and decreased signaling of nitric oxide (NO). Icariin improves erectile function via upregulating multitargets in NO/cyclic guanosine monophosphate (NO/cGMP) pathway, which breviscapine accomplishes by downregulating RhoA/Rho-kinase pathway.

AIM

To investigate the effect and mechanism of icariin combined with breviscapine on the erectile function of SHR.

METHODS

Five 12-week-old male Wistar-Kyoto (WKY) rats and 20 age-matched male SHR were evenly randomized into WKY rats control group, SHR control group, icariin-treated group, breviscapine-treated group, and combined treatment group treated by vehicle, icariin, breviscapine, and icariin plus breviscapine, respectively, by gavage for four successive weeks. Maximum intracavernosal pressure/mean arterial pressure (ICPmax/MAP) and the expression of endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), phosphodiesterase type 5 inhibitors (PDE5), and Rho-associated, coiled-coil containing protein kinase 1 and 2 (ROCK1 and ROCK2) in the cavernous tissues were determined.

RESULTS

The ICPmax/MAP in the combined treatment group was significantly increased compared with SHR control group, icariin-treated group, and breviscapine-treated group. The expression of eNOS and nNOS was significantly higher in the combined treatment group than in SHR control group, icariin-treated group, and breviscapine-treated group (P < 0.05). The expression of PDE5 was significantly lower in the icariin-treated group than in SHR control group (P < 0.05). The expression of ROCK1 was significantly lower in the combined treatment group than in other groups (P < 0.05). The expression of ROCK2 was significantly higher in SHR control group than in WKY rats control group, icariin-treated group, and combined treatment group (P < 0.05). Among these groups, the expression of eNOS and nNOS was the strongest, and ROCK1 was the lowest in WKY rats control group.

CONCLUSION

Icariin combined with breviscapine has synergistic effects on erectile function of SHR through different signal pathways.

摘要

简介

自发性高血压大鼠（SHR）的勃起反应受损是由于 RhoA/Rho-激酶信号的增加和一氧化氮（NO）信号的减少引起的。淫羊藿苷通过上调 NO/cGMP 通路中的多种靶点改善勃起功能，而灯盏花素则通过下调 RhoA/Rho-激酶通路来实现。

目的

探讨淫羊藿苷联合灯盏花素对 SHR 勃起功能的影响及其机制。

方法

将 5 只 12 周龄雄性 Wistar-Kyoto（WKY）大鼠和 20 只同龄雄性 SHR 大鼠平均随机分为 WKY 大鼠对照组、SHR 对照组、淫羊藿苷组、灯盏花素组和联合治疗组，分别灌胃给予载体、淫羊藿苷、灯盏花素和淫羊藿苷加灯盏花素，连续 4 周。测定最大海绵体内压/平均动脉压（ICPmax/MAP）和海绵体组织中内皮型一氧化氮合酶（eNOS）、神经元型一氧化氮合酶（nNOS）、磷酸二酯酶 5 抑制剂（PDE5）和 Rho 相关卷曲螺旋蛋白激酶 1 和 2（ROCK1 和 ROCK2）的表达。

结果

联合治疗组的 ICPmax/MAP 明显高于 SHR 对照组、淫羊藿苷组和灯盏花素组。联合治疗组 eNOS 和 nNOS 的表达明显高于 SHR 对照组、淫羊藿苷组和灯盏花素组（P<0.05）。淫羊藿苷组 PDE5 的表达明显低于 SHR 对照组（P<0.05）。联合治疗组 ROCK1 的表达明显低于其他组（P<0.05）。SHR 对照组 ROCK2 的表达明显高于 WKY 大鼠对照组、淫羊藿苷组和联合治疗组（P<0.05）。在这些组中，WKY 大鼠对照组的 eNOS 和 nNOS 的表达最强，ROCK1 的表达最低。