Kang Jung-Ah, Kim Woo-Seok, Park Sung-Gyoo
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea; Bio Imaging Research Centre and Immune Synapse Research Centre, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea.
Immunology. 2014 Dec;143(4):550-9. doi: 10.1111/imm.12333.
The roles of Notch1 and Notch2 in T-cell function have been well studied, but the functional roles of Notch in B cells have not been extensively investigated, except for Notch2 involvement in peripheral marginal zone B-cell differentiation. This study examined the roles of Notch1 in murine primary B cells. During B-cell activation by B-cell receptor ligation, Notch1 was up-regulated while Notch2 was not. In addition, Notch1 up-regulation itself did not contribute to the further activation of B cells, but the Notch ligand was important for Notch1-mediated further B-cell activation. Moreover, Notch1 deficiency significantly decreased B-cell activation and antibody secretion under the presence of Notch ligand. These data suggest that Notch1 is an important mediator for enhancing B-cell activation and antibody secretion by Notch ligand.
Notch1和Notch2在T细胞功能中的作用已得到充分研究，但除了Notch2参与外周边缘区B细胞分化外，Notch在B细胞中的功能作用尚未得到广泛研究。本研究检测了Notch1在小鼠原代B细胞中的作用。在通过B细胞受体连接激活B细胞的过程中，Notch1上调而Notch2未上调。此外，Notch1上调本身并不有助于B细胞的进一步激活，但Notch配体对于Notch1介导的B细胞进一步激活很重要。此外，在存在Notch配体的情况下，Notch1缺陷显著降低了B细胞激活和抗体分泌。这些数据表明，Notch1是Notch配体增强B细胞激活和抗体分泌的重要介质。
