Christianson Mindy S, Zhao Yulian, Shoham Gon, Granot Irit, Safran Anat, Khafagy Ayatallah, Leong Milton, Shoham Zeev
Department of Gynecology and Obstetrics, Division of Reproductive Endocrinology, Johns Hopkins University School of Medicine, Baltimore, MD, USA,
J Assist Reprod Genet. 2014 Aug;31(8):1029-36. doi: 10.1007/s10815-014-0250-z. Epub 2014 Jun 10.
To identify trends in embryo catheter loading and embryo culture techniques performed worldwide.
A retrospective evaluation using the results of a web-based survey, (IVF Worldwide ( www.IVF-worldwide.com ), was performed.
Responses from 265 centers in 71 countries were obtained. Most centers (97 %) preferred a catheter with its orifice on top, with only 3 % preferring a catheter with the orifice on its side; 41 % preferred a catheter marked for clear ultrasound view. The most commonly-reported methods of embryo loading were medium-air-embryo-air-medium (42 %), medium in catheter with embryo at end (20 %) and medium-air-embryo (15 %). In 68 % of centers the final volume of the catheter was up to 0.3 ml, with only 19 % using 0.3-0.5 ml and 1 % using 0.5-0.7 ml. Using reduced oxygen concentrations for embryo culture was divided between those who used it in combination with the two-gas system (34 %) and those who did not use it at all (39 %); 24 % reported using a three-gas system. Most clinics using reduced oxygen concentrations used it throughout the entire culture period. Half of centers (51 %) reported using reduced oxygen concentrations for the entire IVF population while 6 % reserved it only for blastocyst transfer. The use of sequential media was highly dominant with 40 % reporting its use.
确定全球范围内胚胎导管装载和胚胎培养技术的发展趋势。
利用基于网络的调查（IVF Worldwide，网址：www.IVF-worldwide.com）结果进行回顾性评估。
获得了来自71个国家265个中心的回复。大多数中心（97%）更喜欢孔口在顶部的导管，只有3%更喜欢孔口在侧面的导管；41%更喜欢有清晰超声可视标记的导管。最常报告的胚胎装载方法是培养基-空气-胚胎-空气-培养基（42%）、导管末端有胚胎的培养基（20%）和培养基-空气-胚胎（15%）。68%的中心导管的最终体积可达0.3毫升，只有19%使用0.3 - 0.5毫升，1%使用0.5 - 0.7毫升。使用降低氧气浓度进行胚胎培养的情况分为两类，一类是将其与双气系统联合使用（34%），另一类是根本不使用（39%）；24%报告使用三气系统。大多数使用降低氧气浓度的诊所是在整个培养期间都使用。一半的中心（51%）报告对所有体外受精人群都使用降低氧气浓度，而6%仅将其保留用于囊胚移植。序贯培养基的使用占主导地位，40%报告使用了序贯培养基。
