Lu Y Y, Ni F D, Hong G F
Shanghai Institute of Biochemistry, Academia sinica.
Chin J Biotechnol. 1989;5(3):157-60.
Randomly sequencing of a limited number of unidirectional deletion subclones efficiently generated contiguous DNA sequences with some gaps between them. Gap-bridging subclones were identified by agarose gel electrophoresis. Those located at either end of each individual contiguous sequence were used as standard marker molecules; the subclones between them proved to be correctly identified in most cases. A 4064 bp DNA fragment containing the psr gene was sequenced with the strategy described in this paper.
对有限数量的单向缺失亚克隆进行随机测序，有效地生成了连续的DNA序列，但它们之间存在一些缺口。通过琼脂糖凝胶电泳鉴定缺口桥接亚克隆。位于每个单独连续序列两端的那些亚克隆用作标准标记分子；在大多数情况下，它们之间的亚克隆被证明得到了正确鉴定。采用本文所述策略对一个包含psr基因的4064 bp DNA片段进行了测序。
