Bu G Z, Gong M G, Ding C M, Jia X H, Yao S M, Zhang L
Institute of Tobacco Research, Chinese Academy of Agricultural Sciences, Qingzhou.
Chin J Biotechnol. 1989;5(4):213-22.
The interspecific somatic hybrid plants were produced in 1980 by protoplast fusions between N. tabacum and N. rustica, and N. tabacum and N. glauca. The results of this study on the genetic traits and selection of the hybrid progenies for seven years showed that the performances of the somatic hybrids (SH) were typical intermediate forms of the two species involved in each somatic hybridization, and that segregation began in SH3 and (SH)BC2 generations (the SH1 backcrossed to N. tabacum twice). However, stable lines and male sterile lines from SH1 until the present, were also obtained. The fertilities of the hybrid progenies were generally poor. The characteristics of the recurrent parent were accumulated, and the chromosome numbers of the hybrid plants were gradually decreased as the number of backcrossing generations increased. Some new types of tobacco with certain commercial values have been selected for used in the present study. The conclusion reached, based on our results, is that the somatic hybridization technology has now been successfully utilized to create unique breeding lines of tobacco, and represents a powerful, new tool for crop improvement.
种间体细胞杂种植物于1980年通过烟草与黄花烟草、烟草与光烟草之间的原生质体融合培育而成。对杂种后代的遗传特性进行了为期七年的研究和选择，结果表明，体细胞杂种（SH）的表现是每次体细胞杂交所涉及的两个物种的典型中间形式，并且分离现象始于SH3和（SH）BC2代（SH1与烟草回交两次）。然而，从SH1代到现在，也获得了稳定品系和雄性不育系。杂种后代的育性普遍较差。轮回亲本的特征得以积累，随着回交代数的增加，杂种植物的染色体数目逐渐减少。本研究已筛选出一些具有一定商业价值的新型烟草。基于我们的研究结果得出的结论是，体细胞杂交技术现已成功用于培育独特的烟草育种系，是作物改良的一种强大新工具。
