Lorenz Katrin, Rommel Katharina, Mani Jiju, Jin Nan, Hilgendorf Inken, Ho Anthony D, Freund Mathias, Schmitt Michael, Schmitt Anita
Department of Internal Medicine V, University Clinic Heidelberg , Heidelberg , Germany.
Leuk Lymphoma. 2015 Mar;56(3):671-5. doi: 10.3109/10428194.2014.931956. Epub 2014 Jul 9.
Extracorporeal photopheresis (ECP) constitutes a promising treatment for patients with steroid-refractory acute graft-versus-host disease (aGvHD) after allogeneic stem cell transplantation and for patients with graft rejection after solid organ transplantation (SOT). There is an increasing body of evidence that modulation of lymphocyte subsets might play a crucial role in the mechanism of action in ECP. We therefore analyzed immunological effects concomitantly with clinical findings in patients under ECP therapy using multicolor flow cytometry. In a patient with steroid-refractory aGvHD and a patient with progressive bronchiolitis obliterans syndrome (BOS) after double-lung transplantation, clinical responses to ECP therapy were paralleled by an increase of CD4 + CD25hiFoxP3 + regulatory T cells and a decrease of T(EMRA) (CD3 + CD8+ CD45RA+ CD62L+ effector memory T) cells as well as of natural killer (NK)T cells. In summary, immunomonitoring of T cell subsets can elucidate the mechanism of action in ECP.
体外光化学疗法（ECP）对于异基因干细胞移植后患有类固醇难治性急性移植物抗宿主病（aGvHD）的患者以及实体器官移植（SOT）后发生移植物排斥反应的患者而言，是一种很有前景的治疗方法。越来越多的证据表明，淋巴细胞亚群的调节可能在ECP的作用机制中起关键作用。因此，我们使用多色流式细胞术分析了接受ECP治疗的患者的免疫学效应，并结合临床发现进行研究。在一名患有类固醇难治性aGvHD的患者以及一名双肺移植后发生进行性闭塞性细支气管炎综合征（BOS）的患者中，ECP治疗的临床反应与CD4 + CD25hiFoxP3 +调节性T细胞的增加以及T(EMRA)（CD3 + CD8+ CD45RA+ CD62L+效应记忆T）细胞和自然杀伤（NK）T细胞的减少同时出现。总之，对T细胞亚群的免疫监测可以阐明ECP的作用机制。
