Gure Abera, Lara Francisco J, Moreno-González David, Megersa Negussie, del Olmo-Iruela Monsalud, García-Campaña Ana M
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain; Department of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
Talanta. 2014 Sep;127:51-8. doi: 10.1016/j.talanta.2014.03.070. Epub 2014 Apr 3.
A salting-out assisted liquid-liquid extraction (SALLE) combined with capillary high performance liquid chromatography with diode array detector (capillary HPLC-DAD) was proposed for extraction and determination of residues of nine sulfonylurea herbicides (SUHs) in environmental water and banana juice samples. Various parameters affecting the extraction process such as the type and volume of the organic solvent, sample volume, type and amount of salt, pH of the sample and vortex time were optimized. Under optimum conditions, matrix matched calibration curves were established using river water and banana juice samples. Good linear relationships as well as low limits of detection, LODs (0.4-1.3 and 3-13 µg/L) and quantification, LOQs (1.3-4.3 and 10-43 µg/L) were obtained in water and banana juice samples, respectively. The precision (intra- and inter-day) of the peak areas expressed as relative standard deviations (%, RSD), at two concentration levels were below 10 % in both matrices. Recoveries obtained from spiked environmental waters (river water and groundwater) and banana juice samples, at two concentration levels, ranged from 72 to 115%. The results of the analysis revealed that the proposed SALLE-capillary HPLC method is simple, rapid, cheap and environmentally friendly, being successfully applicable for the determination of SUH residues in waters and banana juices.
提出了一种盐析辅助液液萃取（SALLE）结合二极管阵列检测器的毛细管高效液相色谱法（毛细管HPLC-DAD），用于环境水样和香蕉汁样品中9种磺酰脲类除草剂（SUHs）残留的萃取和测定。对影响萃取过程的各种参数进行了优化，如有机溶剂的类型和体积、样品体积、盐的类型和用量、样品的pH值以及涡旋时间。在最佳条件下，使用河水和香蕉汁样品建立了基质匹配校准曲线。在水样和香蕉汁样品中分别获得了良好的线性关系以及较低的检测限（LODs，0.4 - 1.3和3 - 13 μg/L）和定量限（LOQs，1.3 - 4.3和10 - 43 μg/L）。在两个浓度水平下，以相对标准偏差（%，RSD）表示的峰面积精密度（日内和日间）在两种基质中均低于10%。在两个浓度水平下，加标环境水样（河水和地下水）和香蕉汁样品的回收率在72%至115%之间。分析结果表明，所提出的SALLE - 毛细管HPLC方法简单、快速、廉价且环保，成功适用于水和香蕉汁中SUH残留的测定。
