Yamamoto Eiichi, Ishihama Yasushi, Asakawa Naoki
DDS Research, Global Formulation Research, Pharmaceutical Science and Technology Core Function Unit, Eisai Product Creation Systems, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
Department of Molecular & Cellular BioAnalysis Graduate School of Pharmaceutical Sciences Kyoto University, 46-29 Yoshida-shimoadachichou, Sakyo-ku, Kyoto 616-8501, Japan.
Talanta. 2014 Sep;127:219-24. doi: 10.1016/j.talanta.2014.03.060. Epub 2014 Apr 19.
This report describes the application of partially fluorinated carboxylic acids as ion-pairing reagents for basic analytes in high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI-MS) in positive-ion mode. Partially fluoridated carboxylic acids such as difluoroacetic acid, 3,3,3-trifluoropropionic acid and 3,3,3-trifluoromethyl-2-trifluoromethylpropionic acid functioned as volatile paired-ion similarly as trifluoroacetic acid (TFA). These acids provided basic analytes larger retention factor (k) compared to acetic acid or formic acid in LC. The ESI-MS signal strength of analytes with these acids were higher than that of TFA and was analogous to that of acetic acid or formic acid. The performances of partially fluorinated carboxylic acids in LC and ESI-MS for basic analytes were analyzed by multivariate statistical analysis using physicochemical descriptors of acids. Equations obtained in the analysis enabled us the quantitative evaluation of the performance of fluorinated carboxylic acids as ion-pair reagents for basic analytes in LC/ESI-MS.
本报告描述了部分氟化羧酸作为离子对试剂在高效液相色谱-电喷雾电离质谱联用（LC/ESI-MS）正离子模式下用于碱性分析物的应用。二氟乙酸、3,3,3-三氟丙酸和3,3,3-三氟甲基-2-三氟甲基丙酸等部分氟化羧酸作为挥发性配对离子的功能与三氟乙酸（TFA）类似。与LC中的乙酸或甲酸相比，这些酸为碱性分析物提供了更大的保留因子（k）。使用这些酸的分析物的ESI-MS信号强度高于TFA，与乙酸或甲酸的信号强度类似。通过使用酸的物理化学描述符进行多变量统计分析，分析了部分氟化羧酸在LC和ESI-MS中对碱性分析物的性能。分析中获得的方程使我们能够定量评估氟化羧酸作为LC/ESI-MS中碱性分析物的离子对试剂的性能。
