Lucić N, Mahić Zikić A, Lipa I, Seremet M
Reumatizam. 1989;36(1-6):24-30.
IgM rheumatoid factor was detected, beside ELISA test, by Waaler-Rose, Latex agglutination and indirect immunofluorescent test. 107 patients hospitalized in the Clinic for heart diseases and rheumatism, as well as 50 healthy subjects were examined. In the first group were 50 patients with RA, 30 patients with other rheumatic diseases and 27 with nonrheumatic diseases. According to our results there was no significant difference between sensitivity of ELISA test and other laboratory tests used. ELISA test was positive in 94% of cases of RA, 20% in other rheumatic diseases and only in one case (3,7%) of a nonrheumatic disease. Compared with the control group in which there was no specific reaction, Waaler-Rose and immunofluorescent testing showed a nonspecific reaction in 2% and Latex test in 4% of all cases. Although ELISA test was not significantly sensitive as compared with other laboratory methods, according to our results, it has high reproducibility, specificity and precision (it is measured in international units/mL serum), and it allows following of the concentration of rheumatoid factor. Besides, ELISA test can be done on 100 specimen of blood within 6 hours and the results are known the same day.
除酶联免疫吸附测定（ELISA）试验外，还通过瓦勒-罗斯试验、乳胶凝集试验和间接免疫荧光试验检测了IgM类风湿因子。对在心脏病与风湿病诊所住院的107例患者以及50名健康受试者进行了检查。第一组中有50例类风湿关节炎患者、30例其他风湿性疾病患者和27例非风湿性疾病患者。根据我们的结果，ELISA试验与其他所使用的实验室检测方法在敏感性方面没有显著差异。ELISA试验在94%的类风湿关节炎病例中呈阳性，在其他风湿性疾病中为20%，而在非风湿性疾病中仅1例（3.7%）呈阳性。与无特异性反应的对照组相比，瓦勒-罗斯试验和免疫荧光试验在所有病例中的2%出现非特异性反应，乳胶试验在4%出现非特异性反应。虽然与其他实验室方法相比，ELISA试验的敏感性没有显著差异，但根据我们的结果，它具有高重复性、特异性和精密度（以国际单位/毫升血清进行测定），并且能够监测类风湿因子的浓度。此外，ELISA试验可在6小时内对100份血液标本进行检测，且当天就能得到结果。
