Arienzo Alyexandra, Losito Francesca, Bottini Giorgia, Priolisi Francesca Romana, Mari Alberto, Visca Paolo, Antonini Giovanni
Clin Chem Lab Med. 2014 Oct;52(10):1507-11. doi: 10.1515/cclm-2014-0294.
Urinary tract infections (UTIs) are among the most common infectious diseases. RESULTS obtained from conventional microbiological analysis of urine and antibiotic susceptibility testing are available only after a few days, delaying precise diagnosis and appropriate therapy. Micro Biological Survey (MBS) srl (a spin-off of Roma Tre University, Rome, Italy) has developed and patented an automated colorimetric test for rapid bacterial counting. In a preliminary validation study it was demonstrated that the results obtained with the MBS method are equivalent to the results obtained with conventional culture-based microbiological analysis.
In this study, sterile urine samples were artificially contaminated with bacterial species that are most frequently responsible for UTIs. The MBS method was used to evaluate the presence of bacteria and their sensitivity to some of the most commonly used antibiotics in UTIs.
The MBS method was able to detect in a few hours the presence or absence of bacteria at clinically significant concentrations (>105 CFU/mL), and to provide their susceptibility pattern to a limited panel of antibiotics.
The results obtained demonstrate that the MBS point-of-care testing (POCT) device could be developed into a valuable aid for the management of UTIs, possibly addressing more precise diagnosis and appropriate therapy.
尿路感染（UTIs）是最常见的传染病之一。尿液常规微生物分析和抗生素敏感性测试的结果只有在几天后才能获得，这延迟了精确诊断和适当治疗。微生物调查（MBS）公司（意大利罗马第三大学的衍生公司）开发了一种用于快速细菌计数的自动比色测试并获得了专利。在一项初步验证研究中，结果表明使用MBS方法获得的结果与基于传统培养的微生物分析获得的结果相当。
在本研究中，无菌尿液样本被人工污染了最常导致UTIs的细菌种类。使用MBS方法评估细菌的存在及其对UTIs中一些最常用抗生素的敏感性。
MBS方法能够在几小时内检测出临床显著浓度（>105 CFU/mL）的细菌的存在与否，并提供它们对有限几种抗生素的敏感性模式。
获得的结果表明，MBS即时检验（POCT）设备可发展成为管理UTIs的有价值辅助工具，可能有助于实现更精确的诊断和适当的治疗。
