一种产生环戊吡喃酮类化合物的真菌III型聚酮合酶的表达、纯化及结晶
Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones.
作者信息
Yang Dengfeng, Mori Takahiro, Matsui Takashi, Hashimoto Makoto, Morita Hiroyuki, Fujii Isao, Abe Ikuro
机构信息
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
出版信息
Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):730-3. doi: 10.1107/S2053230X14008516. Epub 2014 May 10.
Abstract
CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 Å, β=114.4°.
摘要
米曲霉的CsyB是一种新型III型聚酮合酶，它催化从脂肪酰辅酶A、丙二酰辅酶A和乙酰乙酰辅酶A形成csypyrone B1 [4-(3-乙酰基-4-羟基-2-氧代-2H-吡喃-6-基)丁酸]。在大肠杆菌中表达的重组CsyB通过坐滴气相扩散法结晶。晶体属于P2₁空间群，晶胞参数为a=70.0、b=104.8、c=73.5 Å，β=114.4°。
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