Jin Shengkai, Cheng Tingcai, Jiang Liang, Lin Ping, Yang Qiong, Xiao Yang, Kusakabe Takahiro, Xia Qingyou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Sericulture and Farm Product Processing Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
PLoS One. 2014 Jun 10;9(6):e99200. doi: 10.1371/journal.pone.0099200. eCollection 2014.
The rat sarcoma-extracellular signal regulated kinase mitogen-activated protein kinases pathway, one of the most ancient signaling pathways, is crucial for the defense against Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Sprouty (Spry) proteins can inhibit the activity of this pathway by receptor tyrosine kinases. We cloned and identified a new B. mori gene with a Spry domain similar to the Spry proteins of other organisms, such as fruitfly, mouse, human, chicken, Xenopus and zebrafish, and named it BmSpry. The gene expression analysis showed that BmSpry was transcribed in all of the examined tissues and in all developmental stages from embryo to adult. BmSpry also induced expression of BmNPV in the cells. Our results indicated: (1) the knock-down of BmSpry led to increased BmNPV replication and silkworm larvae mortality; (2) over-expression of BmSpry led to reduced BmNPV replication; and (3) BmSpry regulated the activation of ERK and inhibited BmNPV replication. These results showed that BmSpry plays a crucial role in the antiviral defense of the silkworm both in vitro and in vivo.
大鼠肉瘤-细胞外信号调节激酶丝裂原活化蛋白激酶通路是最古老的信号通路之一，对家蚕核型多角体病毒（BmNPV）感染的防御至关重要。Sprouty（Spry）蛋白可通过受体酪氨酸激酶抑制该通路的活性。我们克隆并鉴定了一个新的家蚕基因，其具有与果蝇、小鼠、人类、鸡、非洲爪蟾和斑马鱼等其他生物的Spry蛋白相似的Spry结构域，并将其命名为BmSpry。基因表达分析表明，BmSpry在所有检测的组织以及从胚胎到成虫的所有发育阶段均有转录。BmSpry还可诱导细胞中BmNPV的表达。我们的结果表明：（1）敲低BmSpry会导致BmNPV复制增加和家蚕幼虫死亡率升高；（2）过表达BmSpry会导致BmNPV复制减少；（3）BmSpry调节ERK的激活并抑制BmNPV复制。这些结果表明，BmSpry在蚕的体外和体内抗病毒防御中均发挥着关键作用。
