Kumar Mukesh, O'Connell Maile, Namekar Madhuri, Nerurkar Vivek R
Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.
Viruses. 2014 Jun 6;6(6):2328-39. doi: 10.3390/v6062328.
Herein we demonstrate that infection of mice with West Nile virus (WNV) Eg101 provides protective immunity against lethal challenge with WNV NY99. Our data demonstrated that WNV Eg101 is largely non-virulent in adult mice when compared to WNV NY99. By day 6 after infection, WNV-specific IgM and IgG antibodies, and neutralizing antibodies were detected in the serum of all WNV Eg101 infected mice. Plaque reduction neutralization test data demonstrated that serum from WNV Eg101 infected mice neutralized WNV Eg101 and WNV NY99 strains with similar efficiency. Three weeks after infection, WNV Eg101 immunized mice were challenged subcutaneously or intracranially with lethal dose of WNV NY99 and observed for additional three weeks. All the challenged mice were protected against disease and no morbidity and mortality was observed in any mice. In conclusion, our data for the first time demonstrate that infection of mice with WNV Eg101 induced high titers of WNV specific IgM and IgG antibodies, and cross-reactive neutralizing antibodies, and the resulting immunity protected all immunized animals from both subcutaneous and intracranial challenge with WNV NY99. These observations suggest that WNV Eg101 may be a suitable strain for the development of a vaccine in humans against virulent strains of WNV.
在此，我们证明用西尼罗河病毒（WNV）Eg101感染小鼠可提供针对WNV NY99致死性攻击的保护性免疫。我们的数据表明，与WNV NY99相比，WNV Eg101在成年小鼠中基本无毒。感染后第6天，在所有感染WNV Eg101的小鼠血清中检测到WNV特异性IgM和IgG抗体以及中和抗体。蚀斑减少中和试验数据表明，感染WNV Eg101的小鼠血清以相似的效率中和WNV Eg101和WNV NY99毒株。感染三周后，用致死剂量的WNV NY99对感染WNV Eg101的小鼠进行皮下或颅内攻击，并再观察三周。所有受攻击的小鼠均受到保护未发病，且未观察到任何小鼠出现发病和死亡情况。总之，我们的数据首次证明，用WNV Eg101感染小鼠可诱导产生高滴度的WNV特异性IgM和IgG抗体以及交叉反应性中和抗体，由此产生的免疫力可保护所有免疫动物免受WNV NY99的皮下和颅内攻击。这些观察结果表明，WNV Eg101可能是一种适合用于开发人类抗WNV强毒株疫苗的毒株。
