McNamara Robert K, Jandacek Ronald, Rider Therese, Tso Patrick, Chu Wen-Jang, Weber Wade A, Welge Jeffrey A, Strawn Jeffrey R, Adler Caleb M, DelBello Melissa P
a Department of Psychiatry and Behavioral Neuroscience, Center for Imaging Research, Division of Bipolar Disorders Research , University of Cincinnati College of Medicine , Cincinnati , OH , USA.
b Department of Pathology , University of Cincinnati , Cincinnati , OH , USA.
Nutr Neurosci. 2016 May;19(4):145-55. doi: 10.1179/1476830514Y.0000000135. Epub 2016 Mar 2.
To use proton magnetic resonance spectroscopy ((1)H MRS) to investigate the effects of fish oil (FO) supplementation on cortical metabolite concentrations in adolescents with major depressive disorder (MDD).
Metabolite concentrations were determined by (1)H MRS in the anterior cingulate cortex and bilateral dorsolateral prefrontal cortex (DLPFC) of adolescents with MDD before and following 10-week open-label supplementation with low (2.4 g/day, n = 7) or high (16.2 g/day, n = 7) dose FO. Depressive symptom severity scores and erythrocyte fatty acid levels were also determined.
Baseline erythrocyte eicosapentaenoic acid (EPA) composition was positively correlated, and arachidonic acid (AA) and the AA/EPA ratio were inversely correlated, with choline (Cho) concentrations in the right DLPFC. Docosahexaenoic acid (DHA) composition was inversely correlated with myo-inositol (mI) concentrations in the left DLPFC. Erythrocyte EPA and DHA composition increased, and AA decreased, significantly following low-dose and high-dose FO supplementation. In the intent-to-treat sample, depressive symptom severity scores decreased significantly in the high-dose group (-40%, P < 0.0001) and there was a trend in the low-dose group (-20%, P = 0.06). There were no significant baseline-endpoint changes in metabolite levels in each voxel. In the low-dose group there were changes with large effect sizes, including a decrease in mI in the left DLPFC (-12%, P = 0.18, d = 0.8) and increases in glutamate + glutamine (Glx) (+12%, P = 0.19, d = 0.8) and Cho (+15%, P = 0.08, d = 1.2) in the right DLPFC. In the high-dose group, there was a trend for increases in Cho in the right DLPFC (+10%, P = 0.09, d = 1.2).
These preliminary data suggest that increasing the LCn-3 fatty acid status of adolescent MDD patients is associated with subtle changes in Glx, mI, and Cho concentrations in the DLPFC that warrant further evaluation in a larger controlled trial.
运用质子磁共振波谱法（¹H MRS）研究补充鱼油（FO）对重度抑郁症（MDD）青少年皮质代谢物浓度的影响。
采用¹H MRS测定MDD青少年在低剂量（2.4克/天，n = 7）或高剂量（16.2克/天，n = 7）FO开放标签补充10周前后前扣带回皮质和双侧背外侧前额叶皮质（DLPFC）的代谢物浓度。还测定了抑郁症状严重程度评分和红细胞脂肪酸水平。
基线红细胞二十碳五烯酸（EPA）组成与右侧DLPFC中的胆碱（Cho）浓度呈正相关，花生四烯酸（AA）和AA/EPA比值与右侧DLPFC中的Cho浓度呈负相关。二十二碳六烯酸（DHA）组成与左侧DLPFC中的肌醇（mI）浓度呈负相关。低剂量和高剂量FO补充后，红细胞EPA和DHA组成显著增加，AA显著降低。在意向性分析样本中，高剂量组抑郁症状严重程度评分显著降低（-40%，P < 0.0001），低剂量组有降低趋势（-20%，P = 0.06）。每个体素的代谢物水平在基线至终点无显著变化。低剂量组有大效应量的变化，包括左侧DLPFC中mI降低（-12%，P = 0.18，d = 0.8），右侧DLPFC中谷氨酸+谷氨酰胺（Glx）升高（+12%，P = 0.19，d = 0.8）和Cho升高（+15%，P = 0.08，d = 1.2）。高剂量组右侧DLPFC中Cho有升高趋势（+10%，P = 0.09，d = 1.2）。
这些初步数据表明，提高青少年MDD患者的长链n-3脂肪酸水平与DLPFC中Glx、mI和Cho浓度的细微变化有关，这值得在更大规模的对照试验中进一步评估。
