Hartenbach Markus, Delker Andreas, Hartenbach Sabrina, Schlichtiger Juli, Niedermoser Sabrina, Wängler Carmen, Wängler Björn, Böning Guido, Gildehaus Franz Josef, Neumaier Klement, Lauber Kirsten, Kraft Klaus, Belka Claus, Hacker Marcus, Meineke Viktor, Bartenstein Peter
Armed Forces Hospital, Oberer Eselsberg 40, 89081, Ulm, Germany,
Mol Imaging Biol. 2014 Dec;16(6):846-53. doi: 10.1007/s11307-014-0755-y.
The aim of this study is to non-invasively assess early, irradiation-induced normal tissue alterations via metabolic imaging with 3'-deoxy-3'-[(18) F]fluorothymidine ([(18) F]FLT).
Twenty-nine male C57BL/6 mice were investigated by [(18) F]FLT positron emission tomography for 7 days after total body irradiation (1, 4, and 8 Gy) versus 'sham' irradiation (0 Gy). Target/background ratios were determined. The imaging results were validated by histology and immunohistochemistry (Thymidine kinase 1, Ki-67).
[(18) F]FLT demonstrated a dose-dependent intestinal accumulation post irradiation. Mean target/background ratio (±standard error) 0 Gy: 1.4 (0.2), 1 Gy: 1.7 (0.1), 4 Gy: 3.1 (0.3), 8 Gy: 4.2 (0.6). Receiver operating characteristic analysis (area under the curve, p value): 0 vs. 1 Gy: 0.81, 0.049; 0 vs. 4 Gy: 1.0, 0.0016; and 0 vs. 8 Gy: 1.0, 0.0020. Immunohistochemistry confirmed the results.
[(18) F]FLT seems to provide dose-dependent information on radiation-induced proliferation in the bowel. This opens the perspective for monitoring therapy-related side-effects as well as assessing, e.g., radiation accident victims.
本研究的目的是通过使用3'-脱氧-3'-[(18)F]氟胸苷([(18)F]FLT)进行代谢成像，非侵入性地评估早期辐射诱导的正常组织改变。
对29只雄性C57BL/6小鼠进行全身照射(1、4和8 Gy)与“假”照射(0 Gy)，然后用[(18)F]FLT正电子发射断层扫描进行7天的研究。测定靶/本底比值。成像结果通过组织学和免疫组织化学(胸苷激酶1、Ki-67)进行验证。
[(18)F]FLT显示照射后肠道内有剂量依赖性积聚。平均靶/本底比值(±标准误差)：0 Gy：1.4(0.2)，1 Gy：1.7(0.1)，4 Gy：3.1(0.3)，8 Gy：4.2(0.6)。受试者操作特征分析(曲线下面积，p值)：0与1 Gy：0.81，0.049；0与4 Gy：1.0，0.0016；0与8 Gy：1.0，0.0020。免疫组织化学证实了结果。
[(18)F]FLT似乎能提供有关辐射诱导肠道增殖的剂量依赖性信息。这为监测治疗相关的副作用以及评估例如辐射事故受害者等开辟了前景。
