Grossmann A, Weiland F, Weiland E
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Autoimmunity. 1989;2(3):201-11. doi: 10.3109/08916938909014684.
Immunocompetent mice infected with LDVAGIA developed autoantibodies against Golgi antigen as early as 6-7 days postinfection preceding anti-viral antibodies for nearly a week. The anti-Golgi antibody titres peaked between two and three weeks postinfection independent of the applied virus dose. Already one week postinfection anti-Golgi autoantibodies were prominent in IgG subclasses IgG2a and b. Spleen cells from these mice were fused with myeloma cells and the culture fluids were screened by indirect immunofluorescence for antibodies reactive with the Golgi antigen of normal cultured cells. A panel of cloned stable antibody-producing hybridomas has been obtained. Some antibodies showed broad cross-species reactivity, recognizing similar antigenic determinants in the Golgi region of mouse, rat and other mammalian cells and also in avian and piscine cells, whereas others recognized determinants in this cell compartment only in mammalian cells and one recognized Golgi antigen only in particular murine tumor cells. From 19 Golgi antibody producing hybridomas 3 secreted IgM-antibodies, 10 synthesized autoantibodies of the subclass IgG2a and 6 of IgG2b. Applied in immunoelectron microscopy mABs decorated the Golgi organelle. A considerable amount of LDVAGIA-induced hybridomas produced antibodies against conserved antigens associated with the cytoskeleton, mitochondria, and the nucleus, LDV-induced monoclonal anti-Golgi antibodies decorated the Golgi area in LDV- and mock-infected macrophages. However, cytoplasmic fluorescence characteristic for LDV-infected cells was not observed indicating that the anti-Golgi autoantibodies do not cross-react with the virus.
感染LDVAGIA的免疫活性小鼠早在感染后6 - 7天就产生了针对高尔基体抗原的自身抗体，比抗病毒抗体早了近一周。抗高尔基体抗体滴度在感染后两到三周达到峰值，与所应用的病毒剂量无关。感染后仅一周，抗高尔基体自身抗体在IgG亚类IgG2a和b中就很显著。将这些小鼠的脾细胞与骨髓瘤细胞融合，通过间接免疫荧光筛选培养液中与正常培养细胞的高尔基体抗原反应的抗体。已获得一组克隆的稳定产生抗体的杂交瘤。一些抗体显示出广泛的跨物种反应性，能识别小鼠、大鼠和其他哺乳动物细胞以及禽类和鱼类细胞的高尔基体区域中相似的抗原决定簇，而其他抗体仅在哺乳动物细胞的这个细胞区室中识别决定簇，还有一种仅在特定的鼠类肿瘤细胞中识别高尔基体抗原。从19个产生高尔基体抗体的杂交瘤中，3个分泌IgM抗体，10个合成IgG2a亚类的自身抗体，6个合成IgG2b亚类的自身抗体。应用于免疫电子显微镜时，单克隆抗体可标记高尔基体细胞器。大量由LDVAGIA诱导产生的杂交瘤产生了针对与细胞骨架、线粒体和细胞核相关的保守抗原的抗体，LDV诱导的单克隆抗高尔基体抗体可标记LDV感染和模拟感染的巨噬细胞中的高尔基体区域。然而，未观察到LDV感染细胞特有的细胞质荧光，这表明抗高尔基体自身抗体不与病毒发生交叉反应。
