Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.
FEBS Open Bio. 2014 Mar 18;4:315-20. doi: 10.1016/j.fob.2014.03.005. eCollection 2014.
Aquaporin-11 (AQP11) is the latest member of the mammalian water channel protein family to be described. Recent in vivo studies have shown that mutation at Cys(227) causes renal failure. However the importance of Cys(227) for the molecular function of AQP11 is largely unknown. In this study, we examined the subcellular localization, water permeability, and multimerization of AQP11 with a mutation at Cys(227). Interestingly, cells expressing the mutants had significantly higher osmotic water permeability. In contrast, the mutation lowered the cell surface expression and multimerization levels. Our observations suggest that Cys(227) is crucial for the proper molecular function of AQP11.
水通道蛋白 11（AQP11）是最近被描述的哺乳动物水通道蛋白家族的最新成员。最近的体内研究表明，Cys（227）突变会导致肾衰竭。然而，Cys（227）对 AQP11 分子功能的重要性在很大程度上尚不清楚。在这项研究中，我们研究了 Cys（227）突变对 AQP11 的亚细胞定位、水通透性和多聚化的影响。有趣的是，表达突变体的细胞具有显著更高的渗透压水通透性。相比之下，该突变降低了细胞表面表达和多聚化水平。我们的观察表明，Cys（227）对 AQP11 的适当分子功能至关重要。
