Sugawara Kazuharu, Okusawa Makoto, Takano Yusaku, Kadoya Toshihiko
Maebashi Institute of Technology.
Anal Sci. 2014;30(6):649-55. doi: 10.2116/analsci.30.649.
Electrodes modified with gallium(III) complexes were constructed to detect ovalbumin (OVA). For immobilization of a gallium(III)-nitrilotriacetate (NTA) complex, the electrode was first covered with collagen film. After the amino groups of the film had reacted with isothiocyanobenzyl-NTA, the gallium(III) was then able to combine with the NTA moieties. Another design featured an electrode cast with a gallium(III)-acetylacetonate (AA) complex. The amount of gallium(III) in the NTA complex was equivalent to one-quarter of the gallium(III) that could be utilized from an AA complex. However, the calibration curves of OVA using gallium(III)-NTA and gallium(III)-AA complexes were linear in the ranges of 7.0 × 10(-11) - 3.0 × 10(-9) M and 5.0 × 10(-10) - 8.0 × 10(-9) M, respectively. The gallium(III) on the electrode with NTA complex had high flexibility due to the existence of a spacer between the NTA and the collagen film, and, therefore, the reactivity of the gallium(III) to OVA was superior to that of the gallium(III)-AA complex with no spacer.
构建了用镓（III）配合物修饰的电极来检测卵清蛋白（OVA）。为固定镓（III）-次氮基三乙酸（NTA）配合物，首先在电极上覆盖胶原膜。在膜的氨基与异硫氰基苄基-NTA反应后，镓（III）随后能够与NTA部分结合。另一种设计的特点是电极浇铸有镓（III）-乙酰丙酮（AA）配合物。NTA配合物中镓（III）的量相当于可从AA配合物中利用的镓（III）的四分之一。然而，使用镓（III）-NTA和镓（III）-AA配合物的OVA校准曲线分别在7.0×10^(-11) - 3.0×10^(-9) M和5.0×10^(-10) - 8.0×10^(-9) M范围内呈线性。由于NTA与胶原膜之间存在间隔物，带有NTA配合物的电极上的镓（III）具有高柔韧性，因此，镓（III）对OVA的反应性优于没有间隔物的镓（III）-AA配合物。
