Kanai T, Porter J, Gotoh M, Monaco A P, Maki T
Department of Surgery, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215.
Diabetes. 1989 Jan;38 Suppl 1:154-6. doi: 10.2337/diab.38.1.s154.
Elimination or inactivation of lymphoid tissue in the pancreatic islet preparation achieves prolongation of islet-allograft survival. In this study we examined the effect of gamma-irradiation on mouse islet-allograft survival. In a B6AF1 isograft model, irradiation up to 2400 rad did not induce deterioration of islet function over 200 days, but greater doses caused cessation of graft function between 83 and 186 days. When DBA/2 crude islets were transplanted into B6AF1 recipients, all nonirradiated allografts were acutely rejected. Marked prolongation of allograft survival was achieved by islet irradiation with doses between 800 and 12,000 rad. With higher doses, significant numbers of allografts survived beyond the controls, but many lost function between 78 and 180 days, with none surviving greater than 200 days. Irradiation with 16,000 rad caused acute radiation damage. Because most secondary islet allografts in recipient mice that lost primary islet-graft function between 84 and 195 days survived greater than 100 days, late functional loss was probably due to the radiation injury. Combined use of recipient treatment with cyclosporin A and graft irradiation (2400 rad) achieved prolongation of DBA/2 islets in B6AF1 mice.
在胰岛制备过程中消除或灭活淋巴组织可延长胰岛同种异体移植的存活时间。在本研究中，我们检测了γ射线照射对小鼠胰岛同种异体移植存活的影响。在B6AF1同基因移植模型中，高达2400拉德的照射在200天内未导致胰岛功能恶化，但更高剂量会导致移植功能在83至186天之间停止。当将DBA/2粗制胰岛移植到B6AF1受体中时，所有未照射的同种异体移植均被急性排斥。用800至12,000拉德的剂量照射胰岛可显著延长同种异体移植的存活时间。更高剂量时，大量同种异体移植存活时间超过对照组，但许多在78至180天之间失去功能，无一存活超过200天。16,000拉德的照射会导致急性辐射损伤。由于受体小鼠中大多数在84至195天之间失去原发性胰岛移植功能的继发性胰岛同种异体移植存活超过100天，晚期功能丧失可能是由于辐射损伤。受体用环孢素A治疗与移植照射（2400拉德）联合使用可延长B6AF1小鼠中DBA/2胰岛的存活时间。
