Li Hui, Sheng Li-Ping, Wang Bing, Yang Zong-Lin, Liu Shu-Ying
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China
J Chromatogr Sci. 2015 Feb;53(2):285-94. doi: 10.1093/chromsci/bmu056. Epub 2014 Jun 11.
A simple method based on liquid chromatography-full-scan high-resolution accurate mass spectrometry (LC-HRMS) using a quadrupole time-of-flight (TOF) mass spectrometry was developed and optimized for corticosterone quantification in mouse plasma. Mouse plasma (100 μL) was extracted with methyl tert-butyl ether using prednisone as internal standard. Separation was performed on a short C18 column using a methanol-water gradient. Full-scan data were acquired in the TOF only mode, and extracted ion chromatograms were generated post-acquisition with the extract masses of the analytes. Enhanced sensitivity and reproducibility were acquired with optimized mass parameters. The calibration range was 8.24-412 ng/mL, and the limit of quantitation was 5.088 g/mL. Accuracy was between -5.9 and 8.6%. The precision of between-run (interday) and within-run (intraday) was within 5.6 and 6.9%, respectively. The LC-HRMS method was applied for plasma samples analysis from the stressed mice with and without ginseng treatment for the stress state estimation.
