Stadler Jens M, Stafforst Thorsten
Interfaculty Institute of Biochemistry, Auf der Morgenstelle 15. and University of Tübingen, 72076 Tübingen, Germany.
Org Biomol Chem. 2014 Jul 28;12(28):5260-6. doi: 10.1039/c4ob00603h.
Applying psoralen interstrand crosslinks for the photoactivation of nucleic acids is a new concept. To find chromophores that can efficiently stimulate crosslink repair we screened several pyrenes and appended them to peptide nucleic acids for their site-selective addressing. Even though pyrenes conjugated to uracil revealed desirable spectroscopic properties they were not effective in crosslink reversal. In contrast, bare pyrenes are well suitable for crosslink repair with 350 nm light showing an uncaging efficiency similar to classical photocaging groups.
应用补骨脂素链间交联进行核酸的光激活是一个新概念。为了找到能够有效刺激交联修复的发色团，我们筛选了几种芘并将它们连接到肽核酸上以实现位点选择性靶向。尽管与尿嘧啶共轭的芘表现出理想的光谱性质，但它们在交联逆转方面并不有效。相比之下，裸芘非常适合用350纳米光进行交联修复，其解笼效率与经典的光笼基团相似。
