缺氧诱导因子对卵巢癌免疫微环境的影响。
Influence of hypoxia inducible factors on the immune microenvironment in ovarian cancer.
机构信息
Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland
Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, Poland.
出版信息
Anticancer Res. 2014 Jun;34(6):2811-9.
BACKGROUND
Ovarian tumors remain immunogenic even at advanced stages, but cancer-induced immunosuppression abrogates immune surveillance. The composition of the immune microenvironment in ovarian tumors was characterized by analyzing selected immunosuppressive factors in specimens from cancer patients. The influence of the hypoxia inducible factors on the immune microenvironment was also addressed.
MATERIALS AND METHODS
Tumor tissue was collected from 21 ovarian cancer patients immediately following tumor excision during surgery. The mRNA expression of selected genes was quantified, and tumor infiltrating leukocytes were characterized by flow cytometry to identify regulatory T-cells, myeloid-derived suppressor cells, and type-2 macrophages.
RESULTS
Overall, a pronounced heterogeneity was found among the analyzed samples. Nevertheless, statistical analysis revealed that the expression of hypoxia inducible factors correlated with the transcription levels of several immunosuppressive molecules.
CONCLUSION
The activity of hypoxia inducible factors contributes to cancer immunosuppression in ovarian cancer patients.
背景
卵巢肿瘤即使在晚期仍具有免疫原性，但癌症引起的免疫抑制会破坏免疫监视。通过分析来自癌症患者标本中的选定免疫抑制因子，对卵巢肿瘤中免疫微环境的组成进行了表征。还研究了缺氧诱导因子对免疫微环境的影响。
材料和方法
在手术过程中，从 21 名卵巢癌患者的肿瘤切除后立即采集肿瘤组织。通过定量聚合酶链反应定量分析选定基因的 mRNA 表达，并通过流式细胞术对肿瘤浸润性白细胞进行表征，以鉴定调节性 T 细胞、髓系来源的抑制细胞和 2 型巨噬细胞。
结果
总体而言，分析的样本中存在明显的异质性。尽管如此，统计分析显示缺氧诱导因子的表达与几种免疫抑制分子的转录水平相关。
结论
缺氧诱导因子的活性有助于卵巢癌患者的癌症免疫抑制。
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