行乳房缩小术的女性乳腺上皮细胞和白细胞中的 DNA 甲基化。

DNA methylation in paired breast epithelial and white blood cells from women undergoing reduction mammoplasty.

机构信息

Division of Biostatistics and Epidemiology, School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA, U.S.A.

Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, U.S.A.

出版信息

Anticancer Res. 2014 Jun;34(6):2985-90.

PMID:24922663

Abstract

BACKGROUND

The extent to which white blood cell (WBC) DNA methylation provides information on the status of breast epithelial cell DNA is unknown.

PATIENTS AND METHODS

We examined the correlation between methylation in Ras-association domain family-1 gene (RASSF1), a tumor-suppressor gene, and methylation in repetitive elements in paired sets of DNA from WBC and breast epithelial cells collected from 32 women undergoing reduction mammoplasty.

RESULTS

We observed no evidence of correlation in methylation levels for ALU, long interspersed nuclear element-1 (LINE1) or juxtacentromeric satellite-2 (SAT2) (r=0.02 for LINE1, p=0.98; r=0.28 for ALU, p=0.12; r=0.26 for SAT2, p=0.17) for matched sets of DNA from WBC and breast epithelial cells. Variability in these markers across individuals and in the same tissue was low. Five women had an average methylation level above 5% for RASSF1 in breast epithelial cell DNA; however, average methylation levels in WBC DNA for these women were all below 1%.

CONCLUSION

Methylation patterns in WBC DNA did not reflect methylation patterns in the breast.

摘要

背景

白细胞（WBC）DNA 甲基化在多大程度上提供了乳腺上皮细胞 DNA 状态的信息尚不清楚。

患者和方法

我们研究了 Ras 相关结构域家族 1 基因（RASSF1）——一种抑癌基因——在 32 名接受乳房缩小术的女性的 WBC 和乳腺上皮细胞的配对 DNA 中重复元件的甲基化与 RASSF1 甲基化之间的相关性。

结果

我们没有观察到白细胞和乳腺上皮细胞的 DNA 中 ALU、长散布核元件 1（LINE1）或着丝粒卫星 2（SAT2）的甲基化水平存在相关性（LINE1 的 r=0.02，p=0.98；ALU 的 r=0.28，p=0.12；SAT2 的 r=0.26，p=0.17）。这些标志物在个体之间和同一组织中的变异性较低。有 5 名女性的乳腺上皮细胞 DNA 中 RASSF1 的平均甲基化水平高于 5%；然而，这些女性的白细胞 DNA 的平均甲基化水平均低于 1%。