尿道分泌物中产青霉素酶淋病奈瑟菌快速检测方法的评估
Evaluation of a method for rapid detection of penicillinase-producing Neisseria gonorrhoeae in urethral exudates.
作者信息
Herve V M, Georges A J, Massanga M, Martin P M
机构信息
Institut Pasteur, Bangui, Central African Republic.
出版信息
J Clin Microbiol. 1989 Jan;27(1):227-8. doi: 10.1128/jcm.27.1.227-228.1989.
A substrate, pyridine-2-azo-p-dimethylaniline cephalosporin (PADAC; Diagnostic Pasteur, Marnes-La-Coquette, France), for detection of penicillinase-producing Neisseria gonorrhoeae (PPNG) on isolated colonies grown on agar was compared with the nitrocefin reference test (Cefinase; Biomerieux, Marcy l'Etoile, France). The PADAC test was also used to detect PPNG directly on urethral exudates. All 38 PPNG-containing and 29 of 30 PPNG-negative urethral pus isolates gave results in agreement with those obtained on cultured isolates. We conclude that direct detection of PPNG in urethral exudates from males is simple, rapid, reliable, and sensitive.
将用于检测在琼脂上生长的分离菌落中产生青霉素酶的淋病奈瑟菌（PPNG）的底物吡啶 - 2 - 偶氮 - 对二甲苯胺头孢菌素（PADAC；法国马恩斯拉科凯特巴斯德诊断公司）与硝基头孢菌素参考试验（头孢菌素酶；法国马赛 - 埃托伊勒生物梅里埃公司）进行了比较。PADAC试验还用于直接检测尿道分泌物中的PPNG。所有38株含PPNG的菌株以及30株PPNG阴性尿道脓性分泌物分离株中的29株，其检测结果与培养分离株的检测结果一致。我们得出结论，直接检测男性尿道分泌物中的PPNG简单、快速、可靠且灵敏。
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